Platelet miRNA-26b down-regulates multidrug resistance protein 4 in patients on chronic aspirin treatment

Journal of Cardiovascular Medicine
Giulio La RosaFilippo Crea

Abstract

Multidrug resistance protein-4 (MRP4) is an ATP binding cassette membrane transporter, actively involved in the efflux of important pharmacological and physiological molecules. Recently, its over-expression has been associated with reduced aspirin (ASA) efficacy after by-pass surgery. MicroRNAs (miRNAs) are small molecules of non-coding RNA involved in the regulation of many physiological and pathophysiological pathways, are abundant in platelets, and can be modulated by several drugs. In the present study, we assessed the role of platelet miRNAs in modulating MRP4 function in response to ASA. MRP4 mRNA expression has been analyzed by RealTime PCR in platelets from patients on chronic ASA treatment versus a control group. A panel of miRNAs was run on the pool of each cohort. MiRNAs validation was performed by RealTime PCR. To verify whether MRP4 is the target of miR-26b also in platelets, miR-26b was transfected in platelet and DAMI cells with miRNA mimic technology. MRP4 expression was evaluated by flow cytometry and western blotting. We observed a higher MRP4 mRNA expression in platelets of patients under ASA treatment compared to the control group (p<0.005). MiR-26b was found significantly down-regulated in patients on ASA t...Continue Reading

References

Mar 19, 2004·Journal of the American College of Cardiology·Fabio M PulcinelliFrancesco Violi
May 17, 2005·Journal of Thrombosis and Haemostasis : JTH·A D MichelsonUNKNOWN Working Group on Aspirin Resistance
Dec 21, 2006·Nature Clinical Practice. Cardiovascular Medicine·Carlo Patrono, Bianca Rocca
Jan 24, 2007·American Heart Journal·Marcel M C HovensMenno V Huisman
Oct 8, 2008·Circulation·John W EikelboomUNKNOWN Clopidogrel for High Atherothrombotic Risk and Ischemic Stabilization, Management and Avoidance (CHARISMA) Investigators
Nov 11, 2010·Current Medicinal Chemistry·M RaffertyJ Dawson
Nov 5, 2013·Biochemical Pharmacology·Svetlana M Markova, Deanna L Kroetz
May 20, 2014·European Journal of Pharmacology·Li-Ming LienKuan-Hung Lin
Jun 7, 2014·British Journal of Clinical Pharmacology·Isabella MassimiFabio M Pulcinelli
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
Sep 1, 2015·Blood·Benoit DecouturePascale Gaussem

❮ Previous
Next ❯

Citations

May 20, 2020·International Journal of Molecular Sciences·Teresa L KrammerMatthias Hackl
Jan 29, 2020·Research and Practice in Thrombosis and Haemostasis·Ben DriverDianne E van der Wal
May 18, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Wei Liu, Yutian Liu
May 10, 2021·Protein and Peptide Letters·Ioannis AngelisVassilios Tsikaris

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.