Platelet mitochondrial dysfunction in critically ill patients: comparison between sepsis and cardiogenic shock

Critical Care : the Official Journal of the Critical Care Forum
Alessandro ProttiLuciano Gattinoni

Abstract

Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with l...Continue Reading

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Citations

Jul 16, 2015·Journal of Critical Care·Leonardo LorenteEduardo Ruiz-Pesini
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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
electron microscopy

Clinical Trials Mentioned

NCT00541827

Software Mentioned

SigmaPlot

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