PMID: 2505842Jul 11, 1989Paper

Platelet plasminogen activator inhibitor: purification and characterization of interaction with plasminogen activators and activated protein C

Biochemistry
W P Fay, W G Owen

Abstract

Plasminogen activator inhibitor (PAI) was purified in active form from porcine platelets under nondenaturing conditions. The purified inhibitor (Mr 47,000) reacts with tissue-type plasminogen activator (t-PA), urokinase (UK), and activated protein C (APC) to yield both SDS-stable complexes and a modified PAI of slightly reduced molecular weight. The second-order rate constants for the inhibition of t-PA and UK by PAI are 3.5 X 10(7) and 3.4 X 10(7) M-1 s-1, respectively. Activated protein C reacts with PAI with a second-order rate constant of 1.1 X 10(4) M-1 s-1. This rate is not accelerated by protein S, phospholipid, and calcium, or heparin. It is concluded that (1) PAI can function as both inhibitor and substrate of its target proteases, (2) if APC promotes fibrinolysis via inactivation of PAI, then APC must be present in concentrations several orders of magnitude greater than t-PA, or the interaction of APC and PAI must be accelerated by presently unknown mechanisms, and (3) in the absence of heparin, platelet PAI is the most rapid inhibitor of APC yet described.

References

May 15, 1979·FEBS Letters·J Stenflo, M Jönsson
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NyD J Loskutoff
Jul 1, 1985·Journal of Clinical Pathology·N A BoothB Bennett
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L A EricksonD J Loskutoff
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Oct 1, 1984·The Journal of Clinical Investigation·L A EricksonD J Loskutoff
Nov 1, 1981·The Journal of Clinical Investigation·P C Comp, C T Esmon

❮ Previous
Next ❯

Citations

Sep 3, 1991·The American Journal of Cardiology·J H ChesebroV Fuster
Jul 1, 1991·Thrombosis Research·R E Weinstein, F J Walker
Dec 16, 1993·The American Journal of Cardiology·J H ChesebroV Fuster
Nov 4, 1991·The American Journal of Cardiology·J H ChesebroV Fuster
May 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·B A KonkleE M Mazur
Oct 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·W P FayW G Owen
Dec 16, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·L Bajzar
May 19, 2006·Pathophysiology of Haemostasis and Thrombosis·R Manjunatha Kini
Mar 16, 2004·British Journal of Haematology·Anton J G Horrevoets
Jul 13, 2006·The Biochemical Journal·R Manjunatha Kini
Aug 1, 1997·Scandinavian Journal of Clinical and Laboratory Investigation·A Nordenhem, B Wiman
Jun 10, 2018·Scientific Reports·Stéphanie G I Polderdijk, James A Huntington
Oct 1, 1992·European Journal of Haematology·E SøftelandH Holmsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.