Platelet receptor desensitization induced by elevated prostacyclin levels causes platelet-endothelial cell adhesion

Journal of the American College of Cardiology
H DariusJ Meyer

Abstract

The purpose of this study was to investigate the role of platelet prostacyclin receptor desensitization in platelet-endothelial cell adhesion. Platelet-endothelial cell adhesion is regulated by endothelial cell-derived mediators, such as prostacyclin and endothelium-derived relaxing factor. Prostacyclin activates platelet adenylate cyclase and augments cyclic adenosine monophosphate formation by way of specific membrane receptors. Platelet exposure to prostacyclin or chemically stable analogs results in a time- and dose-dependent prostacyclin receptor desensitization as it occurs during infusion therapy with prostacyclin analogs or in pathophysiologic situations such as acute myocardial infarction. Adhesion of washed and radiolabeled human platelets stimulated with thrombin to cultured umbilical vein endothelial cells was measured under control conditions and under conditions of platelet prostacyclin receptor desensitization induced by incubation with the prostacyclin analog iloprost (10 to 100 nmol/liter) for 3 h. Thrombin (0.08 to 0.2 U/ml) increased platelet adhesion in a dose-dependent manner from 2.7 +/- 0.3% to 6.4 +/- 0.6% (mean value +/- SEM). Preincubation of platelets resulted in a dose-dependent down-regulation of 3H...Continue Reading

References

Dec 15, 1987·Thrombosis Research·P A ModestiG F Gensini
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Sneddon, J R Vane
Nov 1, 1973·The Journal of Clinical Investigation·L W HoyerJ R Hoyer
Oct 13, 1984·Lancet·G G Neri SerneriG F Gensini
Apr 26, 1984·The New England Journal of Medicine·G A FitzGeraldA R Brash
Apr 1, 1984·The Journal of Clinical Investigation·A I SchaferM A Gimbrone
Feb 1, 1981·The Journal of Clinical Investigation·M R BuchananJ Hirsch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.