Platelet recruitment promotes keratocyte repopulation following corneal epithelial abrasion in the mouse

PloS One
Fong W LamAlan R Burns

Abstract

Corneal abrasion not only damages the epithelium but also induces stromal keratocyte death at the site of injury. While a coordinated cascade of inflammatory cell recruitment facilitates epithelial restoration, it is unclear if this cascade is necessary for keratocyte recovery. Since platelet and neutrophil (PMN) recruitment after corneal abrasion is beneficial to epithelial wound healing, we wanted to determine if these cells play a role in regulating keratocyte repopulation after epithelial abrasion. A 2 mm diameter central epithelial region was removed from the corneas of C57BL/6 wildtype (WT), P-selectin deficient (P-sel-/-), and CD18 hypomorphic (CD18hypo) mice using the Algerbrush II. Corneas were studied at 6h intervals out to 48h post-injury to evaluate platelet and PMN cell numbers; additional corneas were studied at 1, 4, 14, and 28 days post injury to evaluate keratocyte numbers. In WT mice, epithelial abrasion induced a loss of anterior central keratocytes and keratocyte recovery was rapid and incomplete, reaching ~70% of uninjured baseline values by 4 days post-injury but no further improvement at 28 days post-injury. Consistent with a beneficial role for platelets and PMNs in wound healing, keratocyte recovery was...Continue Reading

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Citations

Dec 1, 2020·Platelets·Paula Oneto, Julia Etulain
May 23, 2020·Experimental Eye Research·Gary H F YamVishal Jhanji
Jul 22, 2018·Experimental Eye Research·Peter J GilliesDamien G Harkin
Jun 15, 2021·Frontiers in Pharmacology·Vishal JhanjiGary Hin-Fai Yam
Jul 25, 2021·International Journal of Molecular Sciences·Angie De La CruzRolando E Rumbaut

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