Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering

Journal of Tissue Engineering and Regenerative Medicine
Eva DohleShahram Ghanaati

Abstract

In the context of prevascularization strategies for tissue-engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence repair processes such as wound healing or angiogenesis. The development of autologous injectable platelet-rich fibrin (PRF), which can be generated from peripheral blood in a minimal invasive procedure, fulfils several requirements for clinically applicable cell-based tissue-engineering strategies. During this study, the established co-culture system of OECs and pOBs was mixed with injectable PRF and was cultivated in vitro for 24 h or 7 days. The aim of this study was to analyse whether PRF might have a positive effect on wound healing processes and angiogenic activation of OECs in the co-culture with regard to proinflammatory factors, adhesion molecules and proangiogenic growth factor expression. Histological cell detection revealed the formation of lumina and microvessel-like structures in the PRF/co-culture complexes after 7 days of complex cultivation. Interestingly, the angiogenic activation of OECs was acco...Continue Reading

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Citations

Jun 1, 2018·Annals of Plastic Surgery·Shaoheng XiongChenggang Yi
May 12, 2019·Journal of Biomedical Materials Research. Part a·Amin RahmaniForough Azam Sayahpour
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Oct 26, 2018·Tissue Engineering. Part C, Methods·Shahram GhanaatiCarlos Fernando de Almeida Barros Mourão
Jan 1, 2021·International Journal of Oral Science·Yu XieXiaoxiao Cai
Mar 7, 2021·International Journal of Molecular Sciences·Sarah Al-MaawiShahram Ghanaati
Apr 6, 2021·Frontiers in Bioengineering and Biotechnology·Sebastian BlattPeer W Kämmerer
Dec 14, 2021·Frontiers in Bioengineering and Biotechnology·Sepehr ShafieeHassan Niknejad

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