Platelet-rich fibrin suppresses in vitro osteoclastogenesis

Journal of Periodontology
Zahra KargarpourReinhard Gruber

Abstract

Platelet-rich fibrin (PRF) membranes can preserve alveolar ridge dimension after tooth extraction. Thus, it can be presumed that PRF suppresses the catabolic events that are caused by osteoclastic bone resorption. To address this possibility, we investigated the impact of soluble extracts of PRF membranes on in vitro osteoclastogenesis in murine bone marrow cultures. Osteoclastogenesis was induced by exposing murine bone marrow cultures to receptor activator of nuclear factor kappa B ligand (RANKL), macrophage colony-stimulating factor (M-CSF) and transforming growth factor-beta 1 (TGF-β1) in the presence or absence of PRF. Osteoclastogenesis was evaluated based on histochemical, gene expression, and resorption analysis. Viability was confirmed by formation of formazan crystals, live-dead staining and caspase-3 activity assay. We report here that in vitro osteoclastogenesis is greatly suppressed by soluble extracts of PRF membranes as indicated by tartrate-resistant acid phosphatase (TRAP) staining and pit formation. In support of the histochemical observations, soluble extracts of PRF membranes decreased expression levels of the osteoclast marker genes TRAP, Cathepsin K, dendritic cell-specific transmembrane protein (DCSTAMP),...Continue Reading

References

Nov 4, 2000·The Journal of Clinical Endocrinology and Metabolism·C DemetercoA Hayek
Sep 29, 2004·The Journal of Experimental Medicine·Toshio KukitaHisayuki Nomiyama
Feb 5, 2005·Journal of Clinical Periodontology·Mauricio G Araújo, Jan Lindhe
Jan 9, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Elisabetta CenniNicola Baldini
May 25, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hermann AgisReinhard Gruber
May 17, 2014·International Journal of Molecular Sciences·Qi LiThomas G H Diekwisch
Jan 27, 2016·Clinical Oral Investigations·Eizaburo KobayashiRichard J Miron
Sep 28, 2016·Tissue Engineering. Part B, Reviews·Richard J MironJoseph Choukroun
Feb 10, 2017·Transfusion·Gabriel StrandbergDavid Berglund
Oct 12, 2018·Clinical Oral Implants Research·Franz Josef StraussReinhard Gruber
Nov 15, 2018·The International Journal of Oral & Maxillofacial Implants·Walter Suruagy Motta PadilhaElizabeth Ferreira Martinez
Aug 4, 2019·Journal of Periodontology·Jila NasirzadeReinhard Gruber

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Citations

Aug 27, 2019·Journal of Biomedical Materials Research. Part a·Jinglun ZhangYufeng Zhang
Jun 25, 2020·Scientific Reports·Francesca Di SummaFranz-Josef Strauss
Oct 21, 2020·International Journal of Molecular Sciences·Zahra KargarpourReinhard Gruber
Apr 27, 2021·Frontiers in Immunology·Zahra KargarpourReinhard Gruber
Sep 22, 2020·Journal of Oral Biology and Craniofacial Research·Amira M Elsherbini, Samah K Ezzat

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