Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model

International Journal of Molecular Sciences
Hye-Rim LeeSun Hee Do

Abstract

Despite the susceptibility to frequent intrinsic and extrinsic injuries, especially in the inner zone, the meniscus does not heal spontaneously owing to its poor vascularity. In this study, the effect of platelet-rich plasma (PRP), containing various growth factors, on meniscal mechanisms was examined under normal and post-traumatic inflammatory conditions. Isolated primary meniscal cells of New Zealand white (NZW) rabbits were incubated for 3, 10, 14 and 21 days with PRP(-), 10% PRP (PRP(+)), IL(+) or IL(+)PRP(+). The meniscal cells were collected and examined using reverse-transcription polymerase chain reaction (RT-PCR). Culture media were examined by immunoblot analyses for matrix metalloproteinases (MMP) catabolic molecules. PRP containing growth factors improved the cellular viability of meniscal cells in a concentration-dependent manner at Days 1, 4 and 7. However, based on RT-PCR, meniscal cells demonstrated dedifferentiation, along with an increase in type I collagen in the PRP(+) and in IL(+)PRP(+). In PRP(+), the aggrecan expression levels were lower than in the PRP(-) until Day 21. The protein levels of MMP-1 and MMP-3 were higher in each PRP group, i.e., PRP(+) and IL(+)PRP(+), at each culture time. A reproducible ...Continue Reading

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Citations

Apr 12, 2017·American Journal of Physical Medicine & Rehabilitation·Zhongwei XuAiguo Zhou
Oct 24, 2017·Frontiers of Medicine·Isabel Andia, Michele Abate
May 2, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Christian O'DonnellConstance R Chu
Jul 1, 2020·Orthopaedic Journal of Sports Medicine·John W BelkEric C McCarty
Oct 9, 2020·Acta Biomaterialia·Solaiman TarafderChang H Lee
Jun 3, 2021·International Journal of Molecular Sciences·Dawid SzwedowskiSławomir Jeka

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Methods Mentioned

BETA
ELISA
PCR
X-ray
biopsy

Software Mentioned

GraphPad
Prism
ImageJ

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