Platelet-Rich Plasma Inhibits Mechanically Induced Injury in Chondrocytes

Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
Xuetao XieRocky S Tuan

Abstract

To investigate the effect of platelet-rich plasma (PRP) on mechanically injured chondrocytes. PRP from bovine whole blood was activated to prepare platelet-rich plasma releasate (PRPr). Bovine articular chondrocytes were subjected to 16%, 0.5-Hz biaxial cyclic tensile strain (CTS) for 48 hours and cultured for another 24 hours without cell stretching as an in vitro model of mechanically injured chondrocytes. Culture medium in the 3 PRP- and CTS-treated groups was supplemented with 10% PRPr at the start of CTS, after 24 hours of CTS, and after 48 hours of CTS, respectively. Gene expression levels of type II collagen, aggrecan, matrix metalloproteinase (MMP)-3, MMP-13, inducible nitric oxide synthase, and cyclooxygenase 2 were quantitatively evaluated. Changes in the content of nitric oxide (NO), prostaglandin E2 (PGE2), MMP-3, and tissue inhibitor of metalloproteinase 1 in the culture medium were also measured. PRPr increased type II collagen and aggrecan messenger RNA expression; diminished CTS-dependent up-regulation of MMP-3, inducible nitric oxide synthase, and cyclooxygenase 2 gene expression; and reduced CTS-induced overproduction of NO and PGE2 when PRPr was applied early at the start of CTS. The addition of PRPr after 24...Continue Reading

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Citations

May 6, 2016·Der Orthopäde·R LenzT Tischer
Jun 16, 2016·The British Journal of Radiology·Antonio BarileCarlo Masciocchi
Sep 11, 2016·Stem Cell Research & Therapy·Heidi R Hofer, Rocky S Tuan
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May 29, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ali FotouhiLeili Aghebati-Maleki
May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Gülseren Irmak, Menemşe Gümüşderelioğlu

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