Abstract
To investigate the effect of platelet-rich plasma (PRP) on mechanically injured chondrocytes. PRP from bovine whole blood was activated to prepare platelet-rich plasma releasate (PRPr). Bovine articular chondrocytes were subjected to 16%, 0.5-Hz biaxial cyclic tensile strain (CTS) for 48 hours and cultured for another 24 hours without cell stretching as an in vitro model of mechanically injured chondrocytes. Culture medium in the 3 PRP- and CTS-treated groups was supplemented with 10% PRPr at the start of CTS, after 24 hours of CTS, and after 48 hours of CTS, respectively. Gene expression levels of type II collagen, aggrecan, matrix metalloproteinase (MMP)-3, MMP-13, inducible nitric oxide synthase, and cyclooxygenase 2 were quantitatively evaluated. Changes in the content of nitric oxide (NO), prostaglandin E2 (PGE2), MMP-3, and tissue inhibitor of metalloproteinase 1 in the culture medium were also measured. PRPr increased type II collagen and aggrecan messenger RNA expression; diminished CTS-dependent up-regulation of MMP-3, inducible nitric oxide synthase, and cyclooxygenase 2 gene expression; and reduced CTS-induced overproduction of NO and PGE2 when PRPr was applied early at the start of CTS. The addition of PRPr after 24...Continue Reading
References
Apr 1, 1990·Trends in Genetics : TIG·L M Matrisian
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·U Benbow, C E Brinckerhoff
Apr 23, 1999·Journal of Biomechanics·D A NarmonevaL A Setton
May 15, 2003·Arthritis and Rheumatism·Parth PatwariAlan J Grodzinsky
May 29, 2004·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M MatsukawaC Hamanishi
Jun 20, 2006·The Journal of Biological Chemistry·Jonathan B FitzgeraldAlan J Grodzinsky
Oct 5, 2007·Gene·J HuangK A Hasty
Jun 13, 2009·Arthritis Research & Therapy·Mary B Goldring, Kenneth B Marcu
Aug 27, 2010·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Giuseppe FilardoMaurilio Marcacci
Nov 11, 2010·Annals of the New York Academy of Sciences·Hui B Sun
Nov 23, 2010·Osteoarthritis and Cartilage·D WangR S Tuan
Dec 17, 2010·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Mandeep DhillonKamal Bali
Mar 26, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jennifer Woodell-MayJacy Hoeppner
Apr 27, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Donald D AndersonJoseph A Buckwalter
Aug 19, 2011·The American Journal of Sports Medicine·Emily A SundmanLisa A Fortier
Aug 23, 2011·The American Journal of Sports Medicine·Gerben M van BuulGerjo J V M van Osch
Nov 3, 2011·Arthritis·D L BaderT T Chowdhury
Jan 21, 2012·BMC Cancer·Luciana R GomesMari C Sogayar
Jun 29, 2012·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Jeffrey M DeLongAugustus D Mazzocca
Jul 24, 2012·Biomaterials·Xuetao XieChangqing Zhang
Jul 31, 2012·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Mikel SánchezEduardo Anitua
Oct 30, 2012·The American Journal of Sports Medicine·Fabio CerzaMichele Ciuffreda
Jan 10, 2013·The American Journal of Sports Medicine·Sandeep PatelAshish Jain
Jan 31, 2013·Tissue Engineering. Part a·Rui Cruz PereiraChiara Gentili
May 15, 2013·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Niall A SmythJohn G Kennedy
Oct 1, 2013·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Massimo PetreraJohn S Theodoropoulos
May 14, 2014·PloS One·Ji LiuChangqing Zhang
Aug 29, 2014·Arthritis Research & Therapy·Xuetao XieRocky S Tuan
Citations
Mar 2, 2016·Expert Opinion on Biological Therapy·Mikel SanchezSabino Padilla
May 6, 2016·Der Orthopäde·R LenzT Tischer
Jun 16, 2016·The British Journal of Radiology·Antonio BarileCarlo Masciocchi
Sep 11, 2016·Stem Cell Research & Therapy·Heidi R Hofer, Rocky S Tuan
Oct 27, 2015·BMC Musculoskeletal Disorders·Tomas MickeviciusArvydas Usas
Mar 22, 2020·PM & R : the Journal of Injury, Function, and Rehabilitation·Michael R BariaSushmitha Durgam
Aug 25, 2018·BioMed Research International·Fan YangChangqing Zhang
Jun 27, 2019·Current Pharmaceutical Biotechnology·Lucía Gato-CalvoFrancisco J Blanco
Feb 6, 2020·JBJS Reviews·Luciano A RossiShane A Shapiro
May 29, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ali FotouhiLeili Aghebati-Maleki
May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Gülseren Irmak, Menemşe Gümüşderelioğlu
Jun 27, 2021·Molecular Medicine·Haijun ZhaoChengkai Shen