Platelet-rich plasma (PRP) stimulates the extracellular matrix metabolism of porcine nucleus pulposus and anulus fibrosus cells cultured in alginate beads

Spine
Koji AkedaKoichi Masuda

Abstract

In vitro assessment of the effects of platelet-rich plasma on the extracellular matrix metabolism of porcine intervertebral disc cells. To determine whether platelet-rich plasma is effective in stimulating cell proliferation and extracellular matrix metabolism by porcine disc cells cultured in alginate beads. Platelet-rich plasma is used to accelerate wound healing and tissue regeneration. Activated platelets release multiple growth factors that regulate cell proliferation, differentiation, and morphogenesis. Individual growth factors present in platelet-rich plasma have been demonstrated to affect the metabolism of intervertebral disc cells. Platelet-poor and platelet-rich plasma was isolated from fresh porcine blood using a commercially available platelet concentration system. After preculture for 7 days and serum starvation for 24 hours, the beads containing nucleus pulposus and anulus fibrosus cells were then cultured for another 72 hours in serum-free medium, 10% fetal bovine serum, 10% platelet-poor plasma, or 10% platelet-rich plasma. The synthesis of proteoglycans and collagen, the accumulation of proteoglycans, and the DNA content were biochemically assessed. Platelet-rich plasma had a mild stimulatory effect on cell p...Continue Reading

References

Mar 1, 1991·Spine·J P ThompsonD S Bradford
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Jan 1, 1987·Annual Review of Medicine·R Ross
Jan 1, 1981·Connective Tissue Research·S HolmA Nachemson
Mar 1, 1993·Blood Reviews·P Harrison, E M Cramer
Sep 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R OsadaH Tsuji
Jul 1, 1997·The American Journal of Physiology·G C HaralabopoulosM E Maragoudakis
Jun 25, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·R E MarxK R Georgeff
Jan 12, 1999·Experimental Cell Research·D J AguiarT R Oegema
Jan 13, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H Ishihara, J P Urban
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Y SunR A Kandel
May 9, 2002·The Annals of Thoracic Surgery·Takeshi HiramatsuYoshinori Takanashi
Jun 19, 2002·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Gernot WeibrichWalter E Hitzler
Oct 31, 2002·The Journal of the American Dental Association·Nathan E Carlson, Robert B Roach
Nov 21, 2002·Biochemical Society Transactions·T R Oegema
Jan 11, 2003·International Journal of Oral and Maxillofacial Surgery·A DugrillonH Klüter
Jun 5, 2003·Experimental and Molecular Pathology·Charleata A CarterCynthia J M Kane
Jun 27, 2003·Microvascular Research·Barden ChanVikas P Sukhatme
Aug 16, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K MasudaE Thonar
Dec 24, 2003·International Journal of Oral and Maxillofacial Surgery·B-H ChoiS-H Lee
Dec 24, 2003·Thrombosis and Haemostasis·Eduardo AnituaAlan T Nurden
Jan 20, 2004·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Haisheng LiCody Bünger
Apr 6, 2004·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Giovanni CrovettiAngelo Carabelli
Jun 24, 2004·American Journal of Physical Medicine & Rehabilitation·Yejia ZhangEugene J M A Thonar
Nov 27, 2004·Spine·Koichi MasudaHoward S An

❮ Previous
Next ❯

Citations

Jul 22, 2009·Archives of Orthopaedic and Trauma Surgery·Dimitris N LyrasAthanasios Kotzakaris
Jul 26, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Koichi Masuda, Howard S An
Sep 4, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Harris Pratsinis, Dimitris Kletsas
Nov 14, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Koichi Masuda
Dec 12, 2012·Evidence-based Spine-care Journal·Gregory B GullungRobert A McGuire
Jul 14, 2010·Tissue Engineering. Part a·Robert E C M MoorenPaul J W Stoelinga
Feb 11, 2010·Tissue Engineering. Part B, Reviews·Patrick Vavken, Martha M Murray
Jan 29, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·T N PirvuS Grad
Oct 11, 2013·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Vaclav ProchazkaGiannoula Lakka Klement
Sep 15, 2015·Journal of Cellular Biochemistry·Yurika MuraseSatoshi Kubota
Dec 3, 2014·Advanced Drug Delivery Reviews·S B G BlanquerA A Poot
Jan 28, 2016·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·David LeviEdward Walko
Jun 5, 2012·Clinics in Sports Medicine·Ken R Mautner, Mandy J Huggins
Feb 18, 2016·Stem Cells International·Jeffrey ZeckserJosh Goodwin
Sep 29, 2011·The Orthopedic Clinics of North America·Won C Bae, Koichi Masuda
Aug 25, 2011·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Patrick VavkenMartha M Murray
May 12, 2010·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Konstantinos KazakosDionysios Verettas
Aug 15, 2015·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Matteo FormicaAlberto Di Martino
Jan 27, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Taralyn McCarrel, Lisa Fortier
Jan 9, 2008·Journal of Cellular Physiology·Yoshiteru KajikawaToshikazu Kubo
Nov 16, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lauren V SchnabelLisa A Fortier
Nov 7, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shigeru KobayashiJill Urban
Dec 14, 2011·Journal of Tissue Engineering and Regenerative Medicine·Antje MietschAnita Ignatius
Nov 11, 2011·Journal of Tissue Engineering and Regenerative Medicine·D R PereiraR L Reis
Nov 22, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiao WangAfsie Sabokbar
Apr 10, 2013·Journal of Biomedical Materials Research. Part a·Joana Silva-CorreiaRui L Reis
Dec 18, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ho-Joong KimChoon-Ki Lee
Nov 29, 2015·The Medical Clinics of North America·Nebojsa Nick KnezevicAlan David Kaye
Mar 21, 2013·Journal of Tissue Engineering and Regenerative Medicine·Gianluca VadalàVincenzo Denaro
Apr 14, 2016·International Orthopaedics·Michael MonfettGregory Lutz
May 13, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Keke GuiWangping Yin
Aug 6, 2013·Biotechnology Advances·Joana Silva-CorreiaRui L Reis
Jan 1, 2012·Stem Cells International·Umile Giuseppe LongoVincenzo Denaro
Dec 17, 2010·The Journal of Bone and Joint Surgery. American Volume·Louay FallouhKazuhisa Takahashi
Jun 3, 2011·The American Journal of Sports Medicine·Nasim Zargar BaboldashtiPhilippa A Hulley
Dec 27, 2016·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·Mohammad NikkhooKinda Khalaf
Dec 27, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Gianluca VadalàVincenzo Denaro
Feb 20, 2018·Tissue Engineering. Part C, Methods·Anita KrouwelsLaura B Creemers
Jan 23, 2018·Regenerative Medicine·Eduardo Anitua, Sabino Padilla

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.