Platelet storage induces accelerated desialylation of platelets and increases hepatic thrombopoietin production

Journal of Translational Medicine
Jooyoung ChoHyun Ok Kim

Abstract

Stored platelets undergo deleterious changes, referred to as platelet storage lesions (PSLs), which accelerate the desialylation of platelets and result in their phagocytosis and clearance by hepatic macrophages. Recent studies have reported that Ashwell-Morell receptor binds to desialylated platelets, thereby inducing hepatic thrombopoietin (TPO) production in a mouse model. Therefore, this study aimed to demonstrate these relationships between PSL and hepatic TPO production in human study. Platelet concentrates (PCs) were obtained from 5 healthy volunteers and the remaining were discarded samples from the blood bank. PCs were divided into two halves, and stored either at 22 or 4 °C. Experiments were conducted using serial samples. Desialylation was assessed using flow cytometry, and structural changes were visualized using electron microscopy. Following co-culture of HepG2 cells (HB-8065, ATCC) with isolated platelets, hepatic TPO production was determined using real-time quantitative polymerase chain reaction and the supernatant TPO level was measured using a Luminex kit. For 5 days of storage duration, platelet counts were not influenced by the storage conditions, but the degree of desialylation was proportional to the stor...Continue Reading

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Citations

Sep 28, 2018·Omics : a Journal of Integrative Biology·Jersey Heitor da Silva MauésRommel Mário Rodriguez Burbano
Jan 25, 2020·The Journal of Experimental Medicine·Carsten DeppermannPaul Kubes
Jun 15, 2019·Current Pharmaceutical Design·Eugeny Yu Gusev, Natalia V Zotova
Oct 18, 2019·Cells·Jersey Heitor da Silva MauésRommel Mário Rodriguez Burbano
Aug 19, 2020·Biomolecules·Jersey Heitor da Silva MauésRommel Mário Rodriguez Burbano
Mar 17, 2021·Experimental Hematology & Oncology·Yuwen ChenYingyu Chen

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Methods Mentioned

BETA
scanning
transmission electron microscopy
scanning electron microscopy
light microscopy
Assay
PCR
flow cytometry
electron microscopy

Software Mentioned

StepOne
Excel
SPSS

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