PMID: 9544577Apr 17, 1998Paper

Platelets induce monocyte differentiation in serum-free coculture

Journal of Leukocyte Biology
C AmmonR Andreesen

Abstract

Terminal maturation of blood monocytes (MO) in vitro and in vivo into macrophages (MAC) occurs as a result of interactions with various cell types. To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with lymphocytes and/or with platelets. We found that intact platelets strongly promoted MO maturation under serum-free conditions as evident from the expression of differentiation-dependent antigens and morphology. To further characterize the differentiation-inducing component(s) we prepared membrane and cytosol fractions of platelets. Both fractions could induce MO maturation, comparable to intact platelets. Further centrifugation of the cytosolic fraction revealed that only the pellet of ultracentrifugation, e.g., membrane fragments, could induce MO differentiation. Digestion with either trypsin or neuraminidase could only partially inhibit this effect. The same was true for heat-treated fractions, indicating that this platelet-derived differentiation stimulus is not solely an intact protein. Next we prepared protein and lipid fractions of platelets. Treatment of MO with platelet proteins or platelet lipids clearly showed that only the lipid components were able t...Continue Reading

Citations

Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geling LiHal E Broxmeyer
Aug 6, 2011·Seminars in Immunopathology·Adriana Vieira-de-AbreuGuy A Zimmerman
Aug 5, 2016·Physiological Reviews·Elizabeth A MiddletonGuy A Zimmerman
Jun 28, 2019·Frontiers in Immunology·Lucas Secchim RibeiroBernardo S Franklin
Jul 8, 2020·Materials·Pei-Ching KungNien-Ti Tsou
Aug 16, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Reinhard GruberGeorg Watzek
Nov 16, 2012·Circulation. Heart Failure·Benjamin J WrigleyGregory Y H Lip
Oct 13, 2006·Clinical and Experimental Immunology·N KatohS Kishimoto
Oct 30, 2020·Frontiers in Immunology·Chongxu ShiHans-Joachim Anders
May 6, 2021·International Journal of Molecular Sciences·Anaís MariscalSilvia Vidal
Jul 24, 2002·Experimental Hematology·Roel WillemsDirk R Van Bockstaele
Oct 30, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kathleen DeiterenAnne Marie Lambeir

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.