Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis

Platelets
Qi ZhouHu Hu

Abstract

Osteoarthritis (OA) is the most common age-related degenerative joint disease and platelet-rich plasma (PRP) has been shown to be beneficial in OA. Therefore, in this study, we aimed to investigate the effects of platelets on chondrocytes and the underlying mechanisms. Anabolic and catabolic activity and the proliferation rate of chondrocytes were evaluated after co-culture with platelets. Chondrocyte gene expression was measured by real-time PCR. Chondrocyte protein expression and phosphorylation were measured by western blot. Chondrocytes treated with or without platelets were transplanted into a rat model of OA induced by intra-articular injection of monosodium iodoacetate and the repair of articular cartilage was evaluated macroscopically and histologically. Platelets significantly promoted the proliferation of chondrocytes, while mildly influencing anabolic and catabolic activity. Chondrocytes co-cultured with platelets showed significantly increased production of bone morphogenetic protein 7 (BMP7). The autocrine/paracrine effect of BMP7 was responsible for the increased proliferation of chondrocytes, via the ERK/CDK1/cyclin B1 signaling pathway. Transplantation of platelet-treated chondrocytes showed better cartilage rep...Continue Reading

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Citations

May 5, 2017·Tissue Engineering. Part C, Methods·Seiju HayashiMitsuo Ochi
Jul 29, 2020·International Journal of Molecular Sciences·Marcin Szustak, Edyta Gendaszewska-Darmach
Oct 11, 2017·Frontiers in Pharmacology·Geoffrey Burnstock
Apr 9, 2021·Frontiers in Immunology·Paula Alvarenga BorgesClaudia Farias Benjamim

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