Platelets secrete stromal cell-derived factor 1alpha and recruit bone marrow-derived progenitor cells to arterial thrombi in vivo.

The Journal of Experimental Medicine
Steffen MassbergMeinrad Gawaz

Abstract

The accumulation of smooth muscle and endothelial cells is essential for remodeling and repair of injured blood vessel walls. Bone marrow-derived progenitor cells have been implicated in vascular repair and remodeling; however, the mechanisms underlying their recruitment to the site of injury remain elusive. Here, using real-time in vivo fluorescence microscopy, we show that platelets provide the critical signal that recruits CD34+ bone marrow cells and c-Kit+ Sca-1+ Lin- bone marrow-derived progenitor cells to sites of vascular injury. Correspondingly, specific inhibition of platelet adhesion virtually abrogated the accumulation of both CD34+ and c-Kit+ Sca-1+ Lin- bone marrow-derived progenitor cells at sites of endothelial disruption. Binding of bone marrow cells to platelets involves both P-selectin and GPIIb integrin on platelets. Unexpectedly, we found that activated platelets secrete the chemokine SDF-1alpha, thereby supporting further primary adhesion and migration of progenitor cells. These findings establish the platelet as a major player in the initiation of vascular remodeling, a process of fundamental importance for vascular repair and pathological remodeling after vascular injury.

References

Jul 12, 1996·Science·T G DiacovoU H von Andrian
Oct 11, 1996·Science·M CallejaG Morata
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·P S FrenetteD D Wagner
Mar 31, 2000·Nature Medicine·P Carmeliet
Dec 6, 2000·The Journal of Clinical Investigation·T PonomaryovT Lapidot
Sep 5, 2002·Circulation·David SimperNoel M Caplice
Oct 9, 2002·The Journal of Experimental Medicine·Steffen MassbergMeinrad Gawaz
Jan 8, 2003·The Journal of Experimental Medicine·Steffen MassbergBernhard Nieswandt
Sep 23, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Denisa D Wagner, Peter C Burger
Oct 7, 2003·Nature Medicine·Alexandra MoersStefan Offermanns
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Steffen MassbergMeinrad Gawaz
Nov 18, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas G NührenbergDietlind Zohlnhöfer
Nov 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shuguang JiangWilliam H Fleming
Apr 9, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Junmei Chen, José A López

❮ Previous
Next ❯

Citations

Apr 24, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Harald F LangerMeinrad Gawaz
Apr 9, 2008·Basic Research in Cardiology·Harald F Langer, Meinrad Gawaz
Mar 16, 2012·Basic Research in Cardiology·Jan-Marcus DanielDaniel G Sedding
Aug 25, 2010·Journal of Thrombosis and Thrombolysis·Dorit Leshem-LevEli I Lev
Jul 28, 2010·Journal of Cardiovascular Translational Research·Christopher OmbrelloCraig N Morrell
Aug 3, 2010·Nature Medicine·Steffen MassbergBernd Engelmann
Oct 13, 2006·Nature Reviews. Cancer·Francesco BertoliniRobert S Kerbel
Mar 13, 2009·Nature Reviews. Cancer·Johanna A Joyce, Jeffrey W Pollard
May 26, 2010·Nature Reviews. Nephrology·Ton J RabelinkAnton J van Zonneveld
Sep 4, 2010·Antioxidants & Redox Signaling·Emmanouil Chavakis, Stefanie Dimmeler
Jun 30, 2007·Journal of Neurotrauma·Li LiuJianning Zhang
Jun 12, 2008·Tissue Engineering. Part C, Methods·Brandon D MarkwayMonica T Hinds
Dec 17, 2009·Cardiovascular Research·Alma Zernecke, Christian Weber
Feb 19, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ingrid StrooJaklien C Leemans
Nov 29, 2007·Current Opinion in Hematology·Hal E Broxmeyer
Sep 22, 2007·Current Opinion in Lipidology·Qingbo Xu
Sep 22, 2007·Current Opinion in Lipidology·Stephan LindemannMeinrad Gawaz
Sep 5, 2008·Current Opinion in Lipidology·Mihail Hristov, Christian Weber
Mar 5, 2008·Genes & Development·Jared WelsDavid Lyden
Nov 22, 2008·Journal of Applied Physiology·Tatyana N MilovanovaStephen R Thom
Jul 5, 2012·Bone Marrow Research·Jennifer L GranickDori L Borjesson
Oct 16, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Lisheng ZhangNeil J Freedman
Mar 30, 2012·Circulation Research·Harald F LangerTriantafyllos Chavakis
Aug 30, 2008·American Journal of Respiratory Cell and Molecular Biology·Fernando A BozzaGuy A Zimmerman
Dec 5, 2006·The Journal of Clinical Investigation·Hans-Georg KoppShahin Rafii
Jan 11, 2012·The Journal of Clinical Investigation·Tania N Petruzziello-PellegriniPhilip A Marsden
Dec 14, 2011·Hematology·Craig N Morrell
Jun 6, 2012·Blood·Voahanginirina RandriamboavonjyIngrid Fleming
Aug 2, 2012·Lipids in Health and Disease·Leandro C F LimaSilvana S Meyrelles

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
fluorescence-activated cell sorting
flow cytometry
ELISA
confocal scanning microscopy
PCR
enzyme-linked immunosorbent assay
transfection
scanning electron microscopy

Software Mentioned

LSM510 META

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.