Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability

Journal of Inorganic Biochemistry
Delia PiconeAntonello Merlino

Abstract

The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A.

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Citations

Apr 5, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Giarita FerraroAntonello Merlino
Aug 3, 2016·Inorganic Chemistry·Irene Russo KraussAntonello Merlino
Jan 11, 2017·Chemistry : a European Journal·Antonello MerlinoLuigi Messori
Nov 27, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Maria SerratriceMaria Agostina Cinellu
Jul 14, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Nicola PontilloAntonello Merlino
Oct 12, 2017·Chemical Communications : Chem Comm·L Messori, A Merlino
Feb 19, 2016·Chemical Communications : Chem Comm·Nicola PontilloAntonello Merlino
Jul 15, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Marco CaterinoAntonello Merlino
Jul 19, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Jana HildebrandtAntonello Merlino

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