Platinum spatula versus Mini-tip Culturette in culturing bacterial keratitis

Cornea
K D EpleyJ B Lasky

Abstract

To compare the traditional method of culturing bacterial keratitis (platinum spatula) with the use of a commercially available Mini-tip Culturette (Becton-Dickinson, Cockeysville, MD, U.S.A.). An experimental model of bacterial keratitis was created in rabbit corneas by intrastromal injection of bacteria. Cultures were taken of rabbit corneas with both the Mini-tip Culturette and the platinum spatula. Culture results were compared with corneal colony counts. Humans with community-acquired presumed bacterial keratitis were cultured with both the Mini-tip Culturette and the platinum spatula. The sensitivity and specificity of the Mini-tip Culturette method was determined and compared with the platinum-spatula technique. Rabbit keratitis model: 100% of corneas had established infections by colony count. Each ulcer was culture positive with platinum spatula, moist Mini-tip Culturette, and dry Mini-tip Culturette. Human keratitis: Seven patients had culture-negative keratitis with both the Mini-tip Culturette and the platinum spatula. Five patients were culture positive with both the Mini-tip Culturette and the platinum spatula. One of the positive cultures had growth of multiple organisms by using the platinum spatula but not with ...Continue Reading

Citations

Jul 15, 1999·Journal of Cataract and Refractive Surgery·D S LamZ Wang
Oct 25, 2003·Cornea·Nathan J RudometkinRichmond E Roeske
Jul 5, 2003·Journal of Clinical Microbiology·Stephen B KayeGraham Harvey
Nov 19, 2013·Survey of Ophthalmology·Lindsay A McGrath, Graham A Lee
Sep 18, 2015·The British Journal of Ophthalmology·Stephen KayeTimothy Neal
Feb 1, 2013·Journal of Ophthalmology·Mary E Marquart, Richard J O'Callaghan
Aug 31, 2021·The Ocular Surface·Lawson Ung, James Chodosh

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