Platonin attenuates LPS-induced CAT-2 and CAT-2B induction in stimulated murine macrophages

Acta Anaesthesiologica Scandinavica
C-C ChenC-J Huang

Abstract

Platonin, a cyanine photosensitizing dye, is a potent immunomodulator that suppresses acute inflammation. Platonin not only inhibits interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha production but also improves circulatory failure in septic rats. In addition, platonin reduces plasma nitric oxide (NO) formation during sepsis. However, the effects of platonin on inducible NO synthase (iNOS) and cationic amino-acid transporter (including CAT-2, CAT-2 A, and CAT-2B) expressions during sepsis remain uninvestigated. Five groups of confluent murine macrophages (RAW264.7 cells) were randomly allocated to receive a 1-h pretreatment of one of five doses of platonin (0.1 microM, 1 microM, 10 microM, 100 microM, or 1000 microM) followed by lipopolysaccharide (LPS; 100 ng ml(-1)). For negative, positive, and platonin control, three other groups of cell cultures were randomly allocated to receive phosphate-buffered saline, LPS, or platonin (1000 microM). The cultures were harvested after exposing them to LPS for 18 h or a comparable duration in those groups without LPS. NO production, L-arginine transport, and expression of the relevant enzymes were then evaluated. Platonin significantly attenuated LPS-induced up-regulatio...Continue Reading

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Citations

Jul 10, 2012·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Kuei-Yao HsuChun-Jen Huang
Mar 21, 2015·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Chia-Tse YehChun-Jen Huang
May 18, 2010·The Journal of Surgical Research·Kuei-Yao HsuChun-Jen Huang
Mar 27, 2010·The Journal of Surgical Research·Jie-Jen LeeChun-Jen Huang
Sep 22, 2009·The Journal of Surgical Research·Shih-Ping ChengYu-Jen Chen
Feb 19, 2013·International Immunopharmacology·Annika VolkeVallo Volke
Oct 12, 2010·Resuscitation·Hsi-Ning ChuChun-Jen Huang
Apr 12, 2012·The Journal of Trauma and Acute Care Surgery·Kuen-Bao ChenChun-Jen Huang

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