Platycodin D-induced apoptosis through nuclear factor-kappaB activation in immortalized keratinocytes

European Journal of Pharmacology
Kwang Seok AhnY S Kim

Abstract

Platycodi Radix is the root of Platycodon grandiflorum and it is widely used in the traditional Oriental medicine as an expectorant for pulmonary diseases and a remedy for respiratory disorders. Platycodin D is the major constituent of triterpene saponins in the root. This study investigates apoptosis by platycodin D in immortalized human keratinocytes (HaCaT). Platycodin D-induced apoptosis in HaCaT cells was confirmed by DNA fragmentation, caspase-3 activation, and caspase-8 activation. Platycodin D could activate inhibitor of nuclear factor-kappaB kinase (IKK)-beta in the nuclear factor-kappaB (NF-kappaB) activation of upstream level, but not IKK-alpha. Pretreated-N-tosyl-l-phenylalanine chloromethyl ketone (TPCK), a potent NF-kappaB inhibitor, could suppress the induction of apoptosis and activation of NF-kappaB of HaCaT cells by platycodin D. We also demonstrated that platycodin D-mediated apoptosis of HaCaT cells upregulates Fas receptor and Fas ligand (FasL) expression, but did not exhibit p53 activation. HaCaT cells were also transfected with pFLF1, which preserves the promoter region of Fas receptor gene containing NF-kappaB binding site. On incubation with platycodin D, the NF-kappaB activity related to Fas receptor i...Continue Reading

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Citations

Apr 24, 2010·Journal of Medicinal Food·Ji Sun Yu, An Keun Kim
Sep 21, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hua QinRu Wang
Sep 25, 2008·Photodermatology, Photoimmunology & Photomedicine·Fabrizio AyalaMaria Antonietta Tufano
Jul 26, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Chae Eun LeeMyung-Sunny Kim
Sep 11, 2012·Journal of Cellular Biochemistry·Jaemoo ChunYeong Shik Kim
Feb 11, 2015·Journal of Ethnopharmacology·Le ZhangYanze Liu
Sep 14, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lin ZhangJing-Kui Tian
Sep 18, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Il Hong SonHyung-In Moon
Jul 24, 2014·Preventive Nutrition and Food Science·Elijah NyakudyaYong-Seob Jeong
Mar 21, 2008·International Journal of Cancer. Journal International Du Cancer·Mun-Ock KimGi-Young Kim
Jul 29, 2010·Journal of Cellular Biochemistry·Eun-Ha Joh, Dong-Hyun Kim
Oct 15, 2011·Experimental Dermatology·Eunsun JungDeokhoon Park
Dec 10, 2015·Journal of Cellular and Molecular Medicine·Muhammad KhanTonghui Ma
Jun 28, 2016·Molecular Biology Reports·Balraj Singh Gill Navgeet
Nov 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Musun ParkChang-Eop Kim
Jan 8, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wei-Chung HsuChih-Yang Huang
Apr 13, 2021·Archiv der Pharmazie·Jinyang ShenJiaojiao Li
Jul 17, 2021·Apoptosis : an International Journal on Programmed Cell Death·Chirine El-BabaNadine Darwiche
Nov 28, 2020·Journal of Ethnopharmacology·Seung-Beom ChaJong-Choon Kim

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