PLC and IP3 -evoked Ca2+ release initiate the fast block to polyspermy in Xenopus laevis eggs

The Journal of General Physiology
Katherine L WozniakAnne E Carlson

Abstract

The prevention of polyspermy is essential for the successful progression of normal embryonic development in most sexually reproducing species. In external fertilizers, the process of fertilization induces a depolarization of the egg's membrane within seconds, which inhibits supernumerary sperm from entering an already-fertilized egg. This fast block requires an increase of intracellular Ca2+ in the African clawed frog, Xenopus laevis, which in turn activates an efflux of Cl- that depolarizes the cell. Here we seek to identify the source of this intracellular Ca2+ Using electrophysiology, pharmacology, bioinformatics, and developmental biology, we explore the requirement for both Ca2+ entry into the egg from the extracellular milieu and Ca2+ release from an internal store, to mediate fertilization-induced depolarization. We report that although eggs express Ca2+-permeant ion channels, blockade of these channels does not alter the fast block. In contrast, insemination of eggs in the presence of Xestospongin C-a potent inhibitor of inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release from the endoplasmic reticulum (ER)-completely inhibits fertilization-evoked depolarization and increases the incidence of polyspermy. Inhibition ...Continue Reading

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Citations

Aug 8, 2018·The Journal of General Physiology·Laurinda A Jaffe
Aug 1, 2020·PLoS Biology·Katherine L WozniakAnne E Carlson
Aug 14, 2019·The Journal of General Physiology·Matthew C Trudeau
Jul 18, 2018·The Journal of General Physiology·Katherine L WozniakAnne E Carlson
Aug 29, 2019·Proceedings of the National Academy of Sciences of the United States of America·Anne E Carlson
May 16, 2019·Molecular Reproduction and Development·Katherine L Wozniak, Anne E Carlson
Jul 4, 2019·The Journal of Biological Chemistry·Maiwase TemboAnne E Carlson
Oct 8, 2021·Molecular Reproduction and Development·Mami WatabeYasuhiro Iwao

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