PLD regulates myoblast differentiation through the mTOR-IGF2 pathway

Journal of Cell Science
Mee-Sup Yoon, Jie Chen

Abstract

A mammalian target of rapamycin (mTOR) pathway is essential for the differentiation of cultured skeletal myoblasts in response to growth factor withdrawal. Previously, phospholipase D (PLD) has been found to play a role in cell growth regulation and mitogenic activation of mTOR signaling. However, a role for PLD in the autocrine regulation of myoblast differentiation is not known. Here we show that upon induction of differentiation in mouse C2C12 satellite cells, the expression of both PLD1 and PLD2 is upregulated. C2C12 differentiation is markedly inhibited by 1-butanol, an inhibitor of the PLD-catalyzed transphosphatidylation reaction, and also by the knockdown of PLD1, but not PLD2. Further investigation has revealed that PLD1 is unlikely to regulate myogenesis through modulation of the actin cytoskeleton as previously suggested. Instead, PLD1 positively regulates mTOR signaling leading to the production of IGF2, an autocrine factor instrumental for the initiation of satellite cell differentiation. Furthermore, exogenous IGF2 fully rescues the differentiation defect resulting from PLD1 knockdown. Hence, PLD1 is critically involved in skeletal myogenesis by regulating the mTOR-IGF2 pathway.

References

Jan 1, 1991·Annual Review of Physiology·J R FloriniK A Magri
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S E TollefsenP Rotwein
Aug 14, 1996·Biochemical and Biophysical Research Communications·S YoshimuraY Nozawa
Mar 7, 1997·The Journal of Biological Chemistry·S A CoolicanJ R Florini
Feb 13, 1999·The Journal of Biological Chemistry·R D GrinerW B Bollag
Jul 30, 1999·Biochimica Et Biophysica Acta·J H Exton
Jul 30, 1999·Biochimica Et Biophysica Acta·M A FrohmanA J Morris
Jan 13, 2000·Acta Physiologica Scandinavica·E R Barton-DavisH L Sweeney
May 22, 2001·Molecular and Cellular Biology·Y Kam, J H Exton
Aug 14, 2001·The Journal of Biological Chemistry·E Erbay, J Chen
Sep 18, 2002·Biochemical Pharmacology·Jie Chen, Yimin Fang
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Dec 5, 2003·Current Biology : CB·Yimin FangJie Chen
Dec 10, 2003·The Journal of Cell Biology·Ebru ErbayJie Chen
Aug 18, 2004·Genes & Development·Nissim Hay, Nahum Sonenberg
Aug 24, 2004·The Journal of Biological Chemistry·Tomoko NishiyamaAkira Kudo
Dec 24, 2004·Molecular Biology of the Cell·Hiba KomatiGeorges Némoz
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·T A HornbergerS Chien
Jul 14, 2006·Cellular Signalling·Sang Hoon HaSung Ho Ryu
Jul 18, 2006·Biochemical and Biophysical Research Communications·Mee-Sup YoonJoong-Soo Han
Oct 27, 2006·Biology of Reproduction·Mee-Sup YoonJoong-Soo Han

❮ Previous
Next ❯

Citations

Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Y SunJ Chen
Apr 29, 2011·The Journal of Biological Chemistry·Rami JaafarGeorges Némoz
Nov 2, 2012·The Journal of Biological Chemistry·Yejing Ge, Jie Chen
Apr 12, 2013·Molecular Biology of the Cell·Keren TazatYoav I Henis
Sep 27, 2013·Molecular Biology of the Cell·Mee-Sup Yoon, Jie Chen
Aug 26, 2009·Molecular and Cellular Biology·Guang-Hui LiuLarry Gerace
Oct 2, 2009·American Journal of Physiology. Cell Physiology·Yejing GeJie Chen
Aug 7, 2013·Cell Communication and Signaling : CCS·Rami JaafarGeorges Némoz
Feb 8, 2011·PloS One·Alexander KlegerThomas Seufferlein
Jan 5, 2011·The Journal of Cell Biology·Yejing GeJie Chen
Oct 26, 2011·The Journal of Cell Biology·Mee-Sup YoonJie Chen
Mar 20, 2010·The EMBO Journal·Shayesta SeenundunFrancis Jeffrey Dilworth
Apr 25, 2009·Developmental Biology·Xin-Xin I ZengH Alex Brown
Nov 28, 2014·Molecular Biology of the Cell·Shuzhi TengPing Huang
Oct 31, 2018·Experimental & Molecular Medicine·Mi-Ock BaekMee-Sup Yoon
Jan 24, 2018·Food & Function·Young Jin JangTae Youl Ha
Nov 30, 2016·Journal of Cachexia, Sarcopenia and Muscle·Christopher Lipina, Harinder S Hundal
Jan 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yimin FangAndrzej Bartke
Feb 5, 2021·International Journal of Molecular Sciences·Ana Gomez-LarrauriAlberto Ouro
May 25, 2013·Journal of Cell Science·Yejing GeJie Chen
Nov 21, 2015·Genes & Development·Liam C HuntFabio Demontis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.