Plectin transcript diversity: identification and tissue distribution of variants with distinct first coding exons and rodless isoforms

Genomics
C E ElliottG Wiche

Abstract

Plectin is a widely expressed protein that is very large in size and that has all the attributes of a multifunctional crosslinking and organizing element of the cytoskeleton. It displays a multidomain structure, versatile binding activities, and subcellular localizations that enable it to strengthen cells against mechanical stress forces. Moreover, hereditary gene defects in plectin cause epidermolysis bullosa simplex (EBS)-MD, a severe skin blistering disease with muscular dystrophy. Here we report the analysis of the exonintron organization of the rat plectin gene and the identification of several different isoforms on the transcriptional level. We show that of 35 coding exons identified, 4 serve as alternative first exons splicing into the same successive exon 2, which is the first of 7 exons encoding a highly conserved actin-binding domain. RNase protection mapping of transcripts containing 3 of the identified 4 alternate first exons revealed their coexpression in rat glioma C6 cells and in a series of different rat tissues that we examined. Significant variations in expression levels of first exons indicated the possibility of tissue-specific promoter usage. In addition, plectin splice variants lacking exon 31 (> 3 kb), wh...Continue Reading

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Citations

Jun 12, 2013·Histochemistry and Cell Biology·Maria J CastañónGerhard Wiche
May 8, 2009·The Journal of Biological Chemistry·Julius KostanGerhard Wiche
Feb 13, 2001·Molecular Biology of the Cell·M GonzalesJ C Jones
Dec 12, 2003·Molecular Biology of the Cell·J KosterA Sonnenberg
Nov 10, 1998·Genes & Development·K AndräG Wiche
Jan 26, 2011·Neurology·D SelcenA G Engel
Apr 9, 1999·Biological Chemistry·F A Steinböck, G Wiche
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·C C ChenJ N Wood
Apr 4, 2000·The Journal of Cell Biology·I KarakesisoglouE Fuchs
Feb 20, 2002·The Journal of Investigative Dermatology·Dörte Koss-HarnesTobias Gedde-Dahl
Dec 17, 2003·The Journal of Investigative Dermatology·Alexandra CharlesworthGuerrino Meneguzzi
Jan 19, 2005·The Journal of Investigative Dermatology·Ellen Pfendner, Jouni Uitto
May 15, 2007·Experimental Cell Research·Arnoud Sonnenberg, Ronald K H Liem
Apr 7, 2005·Experimental Dermatology·E PfendnerJ Uitto
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Dec 23, 2014·Journal of Dermatological Science·Ken Natsuga
Apr 29, 2016·Acta Neuropathologica Communications·Lilli WinterRolf Schröder

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