Plectranthus amboinicus leaves stimulate growth of probiotic L. plantarum: evidence for ethnobotanical use in diarrhea

Journal of Ethnopharmacology
J R Shubha, Praveena Bhatt

Abstract

Leaves of Plectranthus amboinicus are consumed in India along with buttermilk (a probiotic source) during pathogen induced diarrhea. This treatment is known to reduce the number of episodes as well as duration of diarrhea. In the background of its ethnobotanical use, the present investigation was carried out to determine whether, apart from having an antimicrobial activity on pathogens, the leaves could possibly also have a positive effect on the beneficial microflora of the gut resulting in accelerated microbial ecological balance. The growth stimulating activity of the hot water extract (HWE) of P. amboinicus leaves on probiotic Lactobacillus plantarum was determined by microbroth dilution technique and viable plate count method in selective medium (MRS) as well as in fermented milk. The ability of the bacteria to utilize the phytoconstituents of HWE primarily phenolic acids and sugars was determined by assaying for phenolic acid decarboxylase by SDS-PAGE and β-galactosidase activity by β-gal ONPG assay. HWE of P. amboinicus leaves inhibited growth of pathogens (Escherichia coli and Salmonella typhimurium) while stimulated the growth of L. plantarum. SDS-PAGE gel showed the presence of phenolic acid decarboxylase enzyme induc...Continue Reading

References

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Citations

Apr 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Greetha ArumugamUma Rani Sinniah
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sylwester ŚlusarczykAdam Matkowski
Dec 15, 2018··Taslim ErsamTheodore Y. K. Lulan

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