Pleiotrophin induces transdifferentiation of monocytes into functional endothelial cells.

Arteriosclerosis, Thrombosis, and Vascular Biology
B G SharifiPrediman K Shah

Abstract

Pleiotrophin (PTN) is a cytokine that is expressed by monocytes/macrophages in ischemic tissues and that promotes neovascularization, presumably by stimulating proliferation of local endothelial cells. However, the effect of PTN on monocytes/macrophages remains unknown. We investigated the role of PTN in regulating the phenotype of monocytes/macrophages. RT-PCR, real-time PCR, and fluorescence-activated cell sorter analysis revealed that the expression of PTN by monocytic cells led to a downregulation of CD68, c-fms, and CD14 monocytic cell markers and an upregulation of FLK-1, Tie-2, vascular endothelial-cadherin, platelet endothelial cell adhesion molecule-1, endothelial NO synthase, von Willebrand factor, CD34, GATA-2, and GATA-3 endothelial cell markers. Fibrin gel assays showed that the treatment of mouse and human monocytic cells with PTN led to the formation of tube-like structures. In vivo studies showed that PTN-expressing monocytic cells incorporated into the blood vessels of the quail chorioallantoic membrane. The intracardial injection of PTN-expressing monocytic cells into chicken embryos showed that cells integrated only into the developing vasculature. Finally, the injection of PTN-expressing monocytes into a mur...Continue Reading

References

Feb 1, 1994·Molecular and Cellular Biology·N Jahroudi, D C Lynch
Aug 10, 2000·Differentiation; Research in Biological Diversity·B Fernandez PujolK Havemann
Jul 27, 2001·Journal of Cellular Physiology·A IshidaE S Wijelath
Sep 5, 2001·Current Opinion in Genetics & Development·J M Slack, D Tosh
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhaoEliezer Huberman
Sep 3, 2005·The Journal of Clinical Investigation·Dontscho Kerjaschki
Sep 3, 2005·The Journal of Clinical Investigation·Kazuichi MaruyamaJ Wayne Streilein

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Citations

Dec 18, 2012·Journal of Thrombosis and Thrombolysis·Guohou HeYanjun Zeng
Aug 14, 2009·The Journal of Biological Chemistry·Haihua SongTakashi Minami
Nov 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fuqiang LiPrediman K Shah
Apr 9, 2008·Current Opinion in Hematology·Pablo Perez-PineraThomas F Deuel
Sep 22, 2007·Current Opinion in Lipidology·Qingbo Xu
May 2, 2013·PloS One·Praveen GovenderRoss Summer
Aug 1, 2009·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Marie-Therese N VinnarsNikos Papadogiannakis
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Melissa HunterClay Marsh
Aug 22, 2006·Expert Opinion on Biological Therapy·Eben AlsbergMelissa J Mahoney
Jul 29, 2008·Connective Tissue Research·Constantinos Mikelis, Evangelia Papadimitriou
Aug 30, 2008·Journal of Cellular and Molecular Medicine·G KrenningM C Harmsen
Jun 30, 2015·The American Journal of Pathology·Amitava DasSashwati Roy
Jan 1, 2010·Cancers·Kristi D LynnRolf A Brekken
Oct 26, 2007·Journal of Cellular Physiology·Christian HeissMatthew L Springer
Dec 8, 2009·The Journal of Pathology·Alan R WatsonKairbaan M Hodivala-Dilke
Sep 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Gina C SchattemanChunhua Jiao
May 19, 2010·Journal of Cellular and Molecular Medicine·R CarmonaR Muñoz-Chápuli
May 18, 2012·Biotechnology and Bioengineering·James D BryersBuddy D Ratner
Sep 7, 2013·The Journal of Pharmacology and Experimental Therapeutics·Jianli NiuPappachan E Kolattukudy
Nov 18, 2008·Journal of Cellular and Molecular Medicine·F JiangG J Dusting
Feb 24, 2011·Journal of Cellular Biochemistry·Constantinos MikelisEvangelia Papadimitriou
Jun 11, 2019·Frontiers in Physiology·Lais Cavalieri ParedesTarcio Teodoro Braga
Jan 11, 2021·Molecular and Cellular Neurosciences·Rachel RiosAlison K Snyder-Warwick
Jan 13, 2021·Tissue Engineering. Part a·Isobel RountreeFabiola Munarin
Apr 9, 2021·Advanced Healthcare Materials·Lars CrawfordBuddy Ratner
Jul 13, 2021·Archives of Medical Research·Paulina María Reyes-MataDaniel Ortuño-Sahagún
Jan 19, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Karen McLean, Ronald J Buckanovich
Jan 27, 2009·Experimental Cell Research·Annalisa Pacilli, Gianandrea Pasquinelli
Oct 6, 2015·The American Journal of Pathology·Chandan K Sen

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