Pleiotropic effects of a mutation in rfaC on Escherichia coli hemolysin.

Infection and Immunity
M E Bauer, R A Welch

Abstract

Several genes involved in the lipopolysaccharide (LPS) biosynthetic pathway have been shown to affect the expression or activity of Escherichia coli hemolysin (Hly), a secreted cytotoxin that is the prototype of the RTX family of toxins. To further study this relationship, E. coli K-12 strains harboring mutations in the LPS biosynthetic genes rfaS, rfaQ, rfaJ, rfaP, and rfaC were transformed with a recombinant plasmid harboring the hlyCABD operon and examined for their effects on extracellular expression and hemolytic activity. A mutation in rfaC that affected both extracellular expression and activity of Hly was studied in greater detail. This mutation led to a growth-phase-dependent decrease up to 16-fold in the steady-state level of extracellular HlyA, although transcription and secretion of HlyA were decreased no more than 2-fold. Specific hemolytic activity in toxin produced from the rfaC mutant strain was significantly reduced, in a growth-phase-dependent manner. With the rfaC gene supplied in trans, both the decreased expression and activity of Hly were restored to wild-type levels. Hly from the rfaC mutant strain exhibited much slower kinetics of hemolysis, a more rapid rate of decay of activity, and greater formation o...Continue Reading

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Citations

Jul 16, 2002·Infection and Immunity·Gábor NagyLevente Emödy
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Dec 20, 2005·Molecular Microbiology·Carlos BalsalobreSun Nyunt Wai
Nov 15, 2006·Infection and Immunity·Lisa E MånssonAgneta Richter-Dahlfors
Sep 14, 2010·Applied and Environmental Microbiology·Alix MichelOlivier Tenaillon
Feb 26, 2000·Infection and Immunity·C L Weingart, A A Weiss
Jul 30, 2004·American Journal of Veterinary Research·Lisle W GeorgeWilliam W Ruehl
Feb 1, 2008·Molecular Biology of the Cell·Travis J WilesMatthew A Mulvey
Sep 12, 2017·Toxins·Nicole Guiso
Aug 25, 2015·Biochimica Et Biophysica Acta·Laura C Ristow, Rodney A Welch
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·P StanleyC Hughes
Jun 24, 2016·Microbiology Spectrum·Rodney A Welch
Nov 18, 2004·Biochimica Et Biophysica Acta·P Delepelaire
May 17, 2011·Veterinary Microbiology·M CrhanovaI Rychlik

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