Pleiotropic Functions and Biological Potentials of Silver Nanoparticles Synthesized by an Endophytic Fungus

Frontiers in Bioengineering and Biotechnology
Radhika ChandankereXianghui Qi

Abstract

In recent years, the biological synthesis of silver nanoparticles (AgNPs) from microorganisms has become an emerging trend for developing biocompatible nanomaterials that finds applications in nano and biomedical sectors. In the present study, we demonstrated a facile, green and eco-friendly method for AgNPs synthesis using the endophytic fungi (Colletotrichum incarnatum DM16.3) isolated from medicinal plant Datura metel and its in vitro antithrombin and cytotoxic activity. At first, biosynthesis of colloidal AgNPs was predicted by visual observation of color change and UV-visible spectra demonstrated specific surface plasmon resonance peak at 420 nm which confirmed the presence of nanoparticles. Microscopic analyses revealed the structure of highly aggregated, spherical and crystalline AgNPs in the diameter range of 5-25 nm. Transform infrared spectroscopy (FT-IR) spectral analysis confirmed the presence of probable biomolecules required for the reduction of silver ions. In vitro evaluation of thrombin activity demonstrates that AgNPs could exert strong inhibition against both thrombin activity (87%) and thrombin generation (84%), respectively. Further, in silico based mechanistic analysis yielded a better insight in understan...Continue Reading

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Citations

May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Zhanqi DongMinhui Pan

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Datasets Mentioned

BETA
KT358850

Methods Mentioned

BETA
scanning electron microscopy
PCR
electrophoresis
electron microscopy
transmission
X-ray
electron diffraction
AFM
Assay
ELISA

Software Mentioned

MDWeb
NTP
GROMACS
BLASTn
PATCHDOCK

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