Pleiotropic phenotypes of a Yersinia enterocolitica flhD mutant include reduced lethality in a chicken embryo model.

BMC Microbiology
Megan K TownsendBirgit M Prüss

Abstract

The Yersinia enterocolitica flagellar master regulator FlhD/FlhC affects the expression levels of non-flagellar genes, including 21 genes that are involved in central metabolism. The sigma factor of the flagellar system, FliA, has a negative effect on the expression levels of seven plasmid-encoded virulence genes in addition to its positive effect on the expression levels of eight of the flagellar operons. This study investigates the phenotypes of flhD and fliA mutants that result from the complex gene regulation. Phenotypes relating to central metabolism were investigated with Phenotype MicroArrays. Compared to the wild-type strain, isogenic flhD and fliA mutants exhibited increased growth on purines and reduced growth on N-acetyl-D-glucosamine and D-mannose, when used as a sole carbon source. Both mutants grew more poorly on pyrimidines and L-histidine as sole nitrogen source. Several intermediates of the tricarboxylic acid and the urea cycle, as well as several dipeptides, provided differential growth conditions for the two mutants. Gene expression was determined for selected genes and correlated with the observed phenotypes. Phenotypes relating to virulence were determined with the chicken embryo lethality assay. The assay ...Continue Reading

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Citations

Sep 11, 2010·Antonie van Leeuwenhoek·Adrianna RaczkowskaKatarzyna Brzostek
Apr 19, 2011·Applied and Environmental Microbiology·Preeti SuleBirgit M Prüss
Apr 28, 2010·Infection and Immunity·Ilse D JacobsenMatthias Brock
Nov 13, 2012·Journal of Bacteriology·Hannah H TusonDouglas B Weibel
Oct 6, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Beate VölkschMatthias Gube
Jun 1, 2018·Medical Mycology Journal·Emmanuel Nnaemeka NnadiGrace Mebi Ayanbimpe
Jul 25, 2017·Avian Pathology : Journal of the W.V.P.A·Ana E BlancoRudolf Preisinger

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS

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