Pleomorphic copper coordination by Alzheimer's disease amyloid-beta peptide

Journal of the American Chemical Society
Simon C DrewKevin J Barnham

Abstract

Numerous conflicting models have been proposed regarding the nature of the Cu(2+) coordination environment of the amyloid beta (Abeta) peptide, the causative agent of Alzheimer's disease. This study used multifrequency CW-EPR spectroscopy to directly resolve the superhyperfine interactions between Cu(2+) and the ligand nuclei of Abeta, thereby avoiding ambiguities associated with introducing point mutations. Using a library of Abeta16 analogues with site-specific (15)N-labeling at Asp1, His6, His13, and His14, numerical simulations of the superhyperfine resonances delineated two independent 3N1O Cu(2+) coordination modes, {N(a)(D1), O, N(epsilon)(H6), N(epsilon)(H13)} (component Ia) and {N(a)(D1), O, N(epsilon)(H6), N(epsilon)(H14)} (component Ib), between pH 6-7. A third coordination mode (component II) was identified at pH 8.0, and simulation of the superhyperfine resonances indicated a 3N1O coordination sphere involving nitrogen ligation by His6, His13, and His14. No differences were observed upon (17)O-labeling of the phenolic oxygen of Tyr10, confirming it is not a key oxygen ligand in the physiological pH range. Hyperfine sublevel correlation (HYSCORE) spectroscopy, in conjunction with site-specific (15)N-labeling, provid...Continue Reading

References

Jan 1, 1982·Annual Review of Biophysics and Bioengineering·J S Hyde, W Froncisz
Jul 17, 1998·Journal of the Neurological Sciences·M A LovellW R Markesbery
Mar 29, 2001·Physiological Reviews·D J Selkoe
Oct 24, 2002·Journal of the American Chemical Society·David M MorganDavid G Lynn
Sep 27, 2003·Alzheimer Disease and Associated Disorders·Ashley I Bush
Jan 14, 2004·Biochemistry·Craig S AtwoodAshley I Bush
Jul 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kevin J BarnhamAshley I Bush
Apr 6, 2005·Biochemistry·Jesse W KarrVeronika A Szalai
Nov 4, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Duilio F RaffaArvi Rauk
Jan 13, 2006·Journal of Molecular Biology·Tong-Lay LauFrances Separovic
May 9, 2006·Biochemical and Biophysical Research Communications·K GaraiS Maiti
Jul 20, 2006·Journal of the American Chemical Society·Liming Hou, Michael G Zagorski
Aug 10, 2006·Dalton Transactions : an International Journal of Inorganic Chemistry·Imre Sóvágó, Katalin Osz
Aug 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Luc GuilloreauPeter Faller
Mar 14, 2007·Journal of the American Chemical Society·Jesse W Karr, Veronika A Szalai
Apr 19, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Simon C DrewAnthony G Wedd
Jul 5, 2008·Biophysical Journal·Victor A StreltsovJoseph N Varghese
Jun 18, 2001·Angewandte Chemie·Jin ZouNaoki Sugimoto

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Citations

Nov 10, 2010·Journal of Computer-aided Molecular Design·Cristina Rodríguez-RodríguezPilar González-Duarte
Jul 20, 2010·Interdisciplinary Sciences, Computational Life Sciences·T MarinoM Pavelka
Sep 17, 2013·Journal of Magnetic Resonance·James S HydeJason W Sidabras
Jul 1, 2011·Accounts of Chemical Research·Simon C Drew, Kevin J Barnham
Jul 17, 2012·Chemical Reviews·Kasper P Kepp
Aug 2, 2011·Inorganic Chemistry·Fatima Bousejra-ElGarahChristelle Hureau
Jul 19, 2012·Inorganic Chemistry·Yamini P GinotraPrasad P Kulkarni
Dec 12, 2012·Inorganic Chemistry·Chandradeep GhoshSomdatta Ghosh Dey
Jan 27, 2011·Journal of the American Chemical Society·Jason M Kowalski, Brian Bennett
Feb 24, 2011·Journal of the American Chemical Society·Sudhakar ParthasarathyYoshitaka Ishii
Oct 10, 2012·Journal of the American Chemical Society·William A GundersonKurt Warncke
Aug 24, 2010·Chemical Communications : Chem Comm·Ye-Ping YuYan-Mei Li
Sep 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Véronique BallandJean-Michel Savéant
Jun 8, 2012·Cold Spring Harbor Perspectives in Medicine·Colin L Masters, Dennis J Selkoe
Apr 17, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Axel AbeleinSebastian K T S Wärmländer
Jul 21, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Christelle HureauPeter Faller
Nov 10, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Bruno AliesPeter Faller
Jan 25, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Cristina SaccoAnne M Spuches
Dec 10, 2015·The Journal of Physical Chemistry. B·Emiliano De SantisSilvia Morante
Nov 13, 2012·Free Radical Biology & Medicine·Scott AytonAshley I Bush
Apr 1, 2017·Journal of Biomolecular Structure & Dynamics·Shaun T MutterJames A Platts
Mar 2, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ran ZhangQiang Li
Jan 28, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·David E GreenChris Orvig
Aug 8, 2014·Chemical Society Reviews·Kevin J Barnham, Ashley I Bush
Jul 7, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Soumya MukherjeeSomdatta Ghosh Dey
Jun 14, 2018·Biopolymers·D GalanteC D'Arrigo

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