Mar 1, 2012

Plexin-B3 interacts with EB-family proteins through a conserved motif

Biochimica Et Biophysica Acta
Piret LahtAndres Veske


Plexins are transmembrane receptors that are highly expressed in the central nervous system. They participate in the patterning of neural connections and regulation of cell adhesion and motility in many cell types. The aim of this study was to characterize novel protein-protein interactions of plexin-B3 intracellular portion. To identify new interactors of plexin-B3 yeast two-hybrid screen was performed. We used GST pull-down and co-immunoprecipitation to verify those results. Deletion mutants were used to map the interacting regions. The physiological relevance of this interaction was assessed with neurite outgrowth assay in Neuro2A cell line. We show that the N-terminal segment of intracellular domain of plexin-B3 interacts with microtubule plus end-binding proteins EB1, EB2 and EB3. The corresponding region in human plexin-A2, B1 and B3 contains the conserved EB-binding motif SxIP and these plexins also associate with EBs indicating the specificity of plexin-EB binding. As to the EB proteins, their N-terminal microtubule-binding domain is dispensable for plexin interaction. Plexin-EB interaction is involved in neurite growth as the synthetic peptide corresponding to the EB-binding region of plexin-B1 increases significantly ...Continue Reading

  • References20
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Mentioned in this Paper

Conserved Sequence
Microtubule-Associated Protein 3
PLXNA2 gene
Cell Motility
Hormone Receptors, Cell Surface
Neurite Outgrowth
Plus End of Microtubule
Gene Deletion Abnormality
Gene Deletion

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