PLGA doping of PCL affects the plastic potential of human mesenchymal stem cells, both in the presence and absence of biological stimuli.

Journal of Biomedical Materials Research. Part a
J M CurranJ A Hunt

Abstract

A range of poly epsilon-caprolactone (PCL) films mixed/doped with poly(lactide-co-glycolide) (PLGA) (65:35) in 0, 10, 20, and 30 wt % were produced, sterilized using ethylene oxide, and analyzed using FTIR. Characterized human mesenchymal stem cells (hMSCs) were cultured in contact with the materials in basal, chondrogenic, and osteogenic medium for time periods up to 28 days, to determine if the materials could induce differentiation of MSC both in the presence and absence of biological stimuli. Viable cell adhesion was analyzed under all conditions. Collagen I, collagen II, sox-9, osteocalcin, osteopontin, osteonectin, and CBFA1 were evaluated at both the mRNA (real-time PCR) and protein production levels (fluorescent immunohistochemistry) and used to identify cell differentiation. Pure PCL and PCL mixed with PLGA demonstrated a chondrogenic potential. Only PCL 8 (80 wt % PCL, 20 wt % PLGA) facilitated osteogenic differentiation of MSCs under osteogenic conditions. This was attributed to the increased hydrophilic nature of the surface allowing sufficient homogeneous cell attachment and the formation of filamentous F-actin in the cells, allowing osteogenic differentiation. Of all materials tested, PCL 7 (70 wt % PCL, 30 wt % P...Continue Reading

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Citations

Mar 17, 2010·Journal of Materials Science. Materials in Medicine·Nguyen Thi Hiep, Byong-Taek Lee
Aug 14, 2012·Journal of Materials Science. Materials in Medicine·Kelen Jorge Rodrigues da CostaMaria Esperanza Cortés
Jan 1, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Sufang HanJianwu Dai
Apr 19, 2016·Journal of Biomedical Materials Research. Part a·Mohammed A YassinKamal Mustafa
Nov 1, 2011·Revista Brasileira De Ortopedia·Alessandra Deise SebbenJefferson Luis Braga da Silva
Jan 12, 2019·Biomaterials Science·Febriyani F R DamanikLorenzo Moroni

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