PLGA nanoparticles for the oral delivery of 5-Fluorouracil using high pressure homogenization-emulsification as the preparation method and in vitro/in vivo studies

Drug Development and Industrial Pharmacy
XueMing LiQiNeng Ping

Abstract

The objective of the present study was to incorporate the hydrophilic anti-cancer drug 5-Fluorouracil(5-FU) into poly(lactide-co-glycolide) (PLGA) nanoparticles(NP) to improve the oral bioavailability. Owing to the high solubility of 5-FU in basic water, the water-in-oil-in-water (w/o/w) emulsification process has been chosen as one of the most appropriate method for the encapsulation of 5-FU, and the ammonia solution was used as the inner aqueous phase solvent to increase the solubility of 5-FU. In order to reach submicron size as well as increasing the grade of monodispersity compared to previous preparation techniques, we prepared 5-FU loaded PLGA-NP by a high-pressure emulsification-solvent evaporation process. The PLGA-NPs were characterized with respect to their morphology, particle size, size distribution, 5-FU encapsulation efficiency, in vitro and in vivo studies in rats. In vitro release of 5-FU from nanoparticles appeared to have two components with an initial rapid release due to the surface associated drug and followed by a slower exponential release of 5-FU, which was dissolved in the core. The in vivo research was studied in male Sprague-Dawley rats after an oral 5-FU dose of 45 mg/kg. Single oral administration ...Continue Reading

References

Oct 31, 1998·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·S McCleanD Brayden
Feb 11, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·T GovenderS S Davis
Jan 5, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Deborah M Schachter, Joachim Kohn
Jun 1, 2002·Journal of Pharmaceutical and Biomedical Analysis·P ArbósJuan M Irache
Jan 29, 2003·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Yvette Niamien KonanEric Allémann
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Carrie S W ChongJohn Samuel
Jul 12, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Monjed ShakwehElias Fattal
Jan 20, 2006·Journal of Microencapsulation·Avinash BudhianKaren I Winey
Jan 31, 2006·Methods : a Companion to Methods in Enzymology·Jasvinder SinghH Oya Alpar

❮ Previous
Next ❯

Citations

May 30, 2015·International Journal of Pharmaceutics·S A GâreaA Sârbu
Sep 17, 2013·Pharmaceutical Development and Technology·Doaa H MobarakSeham A Elkheshen
May 8, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kaushik ThankiSanyog Jain
Feb 11, 2015·Clinical Pharmacokinetics·Kamla Pathak, Smita Raghuvanshi
Feb 14, 2015·European Journal of Medicinal Chemistry·Van-Minh LeJian-Wen Liu
Oct 3, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·José L Arias
Oct 21, 2015·Chemical Communications : Chem Comm·Qinqin ChengYangzhong Liu
Mar 25, 2019·AAPS PharmSciTech·Manoj Kumar GautamRenu Chadha
Jun 5, 2019·Journal of Biomedical Materials Research. Part a·Ting LiJianhua Wang
Feb 27, 2015·Materials·M Biswaranjan SubudhiSanjay K Jain
Oct 19, 2017·Journal of Pharmaceutical Sciences·Nattawut LeelakanokAliasger Salem
Dec 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Zhun Zhou, Dennis Bong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.