PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation

Journal of Cell Science
Susana Montenegro GouveiaMonica Bettencourt-Dias

Abstract

The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critical for the recruitment of several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of Xenopus egg extracts, where PLK4 can induce de novo MTOC formation ( Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that in vitro, PLK4 can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PLK4 assemblies additionally recruit STIL, a substrate of PLK4, and the microtubule nucleator γ-tubulin, forming acentriolar MTOCs de novo The assembly of these robust microtubule asters is independent of dynein, similar to what is found for centrosomes. We suggest a new mechanism of action for PLK4, where it forms a self-organising catalytic scaffold that recruits centriole components, PCM factors and α- and β-tubulins, leading to MTOC formatio...Continue Reading

References

Apr 29, 1992·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T J Mitchison
Jan 1, 1994·Cell Motility and the Cytoskeleton·R Brandt, G Lee
Jan 1, 1993·The Journal of Cell Biology·T UmeyamaN Hirokawa
May 14, 1999·Current Opinion in Structural Biology·C Wiese, Y Zheng
Aug 15, 2002·Current Biology : CB·Andrei V PopovEric Karsenti
Oct 26, 2005·Nature Cell Biology·Robert HabedanckErich A Nigg
Dec 6, 2005·Current Biology : CB·M Bettencourt-DiasD M Glover
Apr 28, 2007·Science·A Rodrigues-MartinsM Bettencourt-Dias
Jan 15, 2008·Cell·Gary J BrouhardAnthony A Hyman
Mar 12, 2009·Molecules and Cells·Jadranka Loncarek, Alexey Khodjakov
Jul 28, 2009·Cell·Srinivas HonnappaMichel O Steinmetz
Aug 10, 2010·Cell·Claire E Walczak, Sidney L Shaw
Jan 29, 2011·Science·Mark van BreugelBenoît Zuber
Mar 1, 2011·Current Biology : CB·Frank EckerdtJames L Maller
Jan 19, 2012·PloS One·Eileen O'TooleThomas Müller-Reichert
Feb 4, 2012·Journal of Cell Science·Ramona BahtzIngrid Hoffmann
Nov 8, 2012·Journal of Cell Science·Neus Teixidó-TravesaJens Lüders
Nov 26, 2013·Developmental Cell·Paula A CoelhoMagdalena Zernicka-Goetz
Feb 18, 2014·Current Opinion in Cell Biology·Swadhin Chandra JanaMónica Bettencourt-Dias
Feb 24, 2015·Biology Open·Anne-Sophie KratzIngrid Hoffmann
Jun 23, 2015·Nature Cell Biology·Michal WieczorekGary J Brouhard
Jun 24, 2015·The Journal of Cell Biology·Tyler C MoyerAndrew J Holland
Jul 16, 2015·Methods in Cell Biology·Dong Kong, Jadranka Loncarek
Jul 16, 2015·Methods in Cell Biology·Jeffrey B Woodruff, Anthony A Hyman
Jul 19, 2015·ELife·Christian ArquintTimm Maier
Oct 21, 2015·Developmental Cell·Carla A M LopesMónica Bettencourt-Dias
Apr 27, 2016·Current Biology : CB·Sihem ZitouniMónica Bettencourt-Dias
May 25, 2016·Developmental Cell·Ashwani SharmaMichel O Steinmetz
Sep 27, 2016·Current Opinion in Cell Biology·Ariana D Sanchez, Jessica L Feldman
Aug 24, 2017·Nature Reviews. Molecular Cell Biology·Johanna Roostalu, Thomas Surrey
Sep 7, 2017·Cell Reports·Amayra Hernández-VegaAnthony A Hyman
Nov 28, 2017·Trends in Cell Biology·Joel Paz, Jens Lüders

❮ Previous
Next ❯

Citations

Jul 13, 2019·Cells·Vladimir Joukov, Arcangela De Nicolo
Jan 3, 2019·Annual Review of Biochemistry·David K Breslow, Andrew J Holland
Sep 11, 2020·Nature·Franz MeitingerKaren Oegema
Feb 23, 2019·Journal of Cell Science·Pierre Gönczy, Georgios N Hatzopoulos
Dec 7, 2020·Cytoskeleton·Joseph Y OngJorge Z Torres
Jan 8, 2021·Frontiers in Cell and Developmental Biology·Max KoppersGinny G Farías
Jan 19, 2021·Seminars in Cell & Developmental Biology·Swadhin Chandra Jana
Mar 25, 2021·The Journal of Cell Biology·Catarina NabaisMónica Bettencourt-Dias
May 8, 2021·Biochemical Society Transactions·Alan Wainman

❮ Previous
Next ❯

Methods Mentioned

BETA
Electron microscopy
Fluorescence
confocal microscopy
PCR

Software Mentioned

ImageJ
MetaMorph
Fiji ( NIH )
Prism
Adobe
GraphPad
Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.