Plumbagin, isolated from Plumbago zeylanica, induces cell death through apoptosis in human pancreatic cancer cells

Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
Chien-An ChenYu-Jen Chen

Abstract

Pancreatic cancer is one of the most resistant malignancies. Several studies have indicated that plumbagin isolated from Plumbago zeylanica possesses anticancer activity. However, its antitumor effects against pancreatic cancer have not been explored. We investigated the effect of plumbagin on the growth of human pancreatic carcinoma cells and its possible underlying mechanisms. Plumbagin inhibited the growth of Panc-1 and Bxpc-3 cells in a dose-dependent and time-dependent manner. Liu's staining and transmission electron microscopy demonstrated morphological changes resembling apoptosis in Panc-1 cells treated with plumbagin. The degree of apoptosis was assessed by measuring the proportions of sub-G(1), annexin V+/propidium iodide-, and terminal- deoxynucleotidyl-transferase-mediated-nick-end labeling (TUNEL)+ cells, and a significant increment in apoptotic cells was observed. Exposure to plumbagin caused the upregulation of Bax, a rapid decline in mitochondrial transmembrane potential, apoptosis-inducing factor overexpression in cytosol, and the cleavage of procaspase-9 and poly ADP-ribose polymerase. Activation of caspase-3, but not caspase-8, was evidenced by fluorometric substrate assay. Pretreatment with caspase inhibitor...Continue Reading

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Citations

Nov 1, 2012·Journal of Natural Products·Adrian LupescuFlorian Lang
Aug 29, 2013·Medical Science Monitor Basic Research·Jia-xuan QiuZong-fu Mao
Apr 17, 2013·Targeted Oncology·Daniela Laura PapademetrioElida Alvarez
Oct 13, 2012·Medicinal Research Reviews·Subhash PadhyeFazlul H Sarkar
Feb 11, 2012·International Journal of Cancer. Journal International Du Cancer·Bilal Bin HafeezAjit Kumar Verma
May 31, 2014·The International Journal of Biochemistry & Cell Biology·Lin Li, Po Sing Leung
Apr 1, 2017·The American Journal of Chinese Medicine·Yuling LiuHui Li
Jan 5, 2018·Artificial Cells, Nanomedicine, and Biotechnology·S RajalakshmiBothiraja Chellampillai
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Solomon TesfayeIlias Muhammad

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