Plumbagin suppresses the migration and invasion of glioma cells via downregulation of MMP-2/9 expression and inaction of PI3K/Akt signaling pathway in vitro

Journal of Pharmacological Sciences
Guanghui ChenBin Xiao

Abstract

Plumbagin is a natural naphthoquinone constituent isolated from the roots of medicinal plant Plumbago zeylanica L., and has demonstrated anti-proliferative and anti-invasion activities in various cancer cells. However, its effect on the migration and invasion of glioma cells has not been elucidated. Therefore, human glioma U87 and U251 cells were treated with plumbagin at 1.0 and 2.0 μM for 24 h, and cell migration and invasion were assessed with scratch wound healing and invasion assays. The results showed that plumbagin significantly inhibited the migration and invasion of U87 and U251 cells, suppressed the activity and expression of MMP-2/-9, and inhibited the nuclear translocation of transcription factors Sp1 in the U87 and U251 cells. Moreover, plumbagin reduced the level of p-PI3K and p-Akt in these cells. The combined treatment with plumbagin and PI3K/Akt agonist insulin-like growth factor-1 (IGF-1) reversed plumbagin-mediated inhibitory effects on MMP-2/-9 expression, cell migration and invasion. These findings suggest that the plumbagin-induced inhibition of glioma cell migration and invasion is closely associated with the downregulation of MMP-2/-9 expression and activity, and suppression of PI3K/Akt signaling pathway...Continue Reading

Citations

Oct 26, 2018·Journal of Cellular and Molecular Medicine·Qianqian ZhengBiao Wang
Apr 26, 2018·Anti-cancer Agents in Medicinal Chemistry·Beatriz L HerasSonsoles Hortelano
May 30, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chiun-Wei HuangFeng-Ting Huang
Dec 18, 2020·Cancer Cell International·Zhiyong WuHao Long
Sep 22, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Li WangKunkun Li
Dec 22, 2020·BioMed Research International·Zhenhua YinWenyi Kang
Apr 23, 2021·The Journal of Pharmacy and Pharmacology·Bishwanath AcharyaKesara Na-Bangchang

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