Embryonic stem cells exhibit pluripotency: they can differentiate into all types of somatic cells. Pluripotent genes such as Oct4 and Nanog are activated in the pluripotent state, and their expression decreases during cell differentiation. Inversely, expression of differentiation genes such as Gata6 and Gata4 is promoted during differentiation. The gene regulatory network controlling the expression of these genes has been described, and slower-scale epigenetic modifications have been uncovered. Although the differentiation of pluripotent stem cells is normally irreversible, reprogramming of cells can be experimentally manipulated to regain pluripotency via overexpression of certain genes. Despite these experimental advances, the dynamics and mechanisms of differentiation and reprogramming are not yet fully understood. Based on recent experimental findings, we constructed a simple gene regulatory network including pluripotent and differentiation genes, and we demonstrated the existence of pluripotent and differentiated states from the resultant dynamical-systems model. Two differentiation mechanisms, interaction-induced switching from an expression oscillatory state and noise-assisted transition between bistable stationary state...Continue Reading
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
Regulated expression of pdx-1 promotes in vitro differentiation of insulin-producing cells from embryonic stem cells
Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages
Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
Multistability and clustering in a population of synthetic genetic oscillators via phase-repulsive cell-to-cell communication
The mixed-lineage kinase 1-3 signalling pathway regulates stress response in cardiac myocytes via GATA-4 and AP-1 transcription factors
A general model for binary cell fate decision gene circuits with degeneracy: indeterminacy and switch behavior in the absence of cooperativity
Systematic dissection of regulatory motifs in 2000 predicted human enhancers using a massively parallel reporter assay
Quantifying Waddington landscapes and paths of non-adiabatic cell fate decisions for differentiation, reprogramming and transdifferentiation
Epigenetics decouples mutational from environmental robustness. Did it also facilitate multicellularity?
Rethinking gene regulatory networks in light of alternative splicing, intrinsically disordered protein domains, and post-translational modifications
HPV16-E2 protein modifies self-renewal and differentiation rate in progenitor cells of human immortalized keratinocytes
Requirements for efficient cell-type proportioning: regulatory timescales, stochasticity and lateral inhibition
SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells
Exploring induced pluripotency in human fibroblasts via construction, validation, and application of a gene regulatory network
A possible role for epigenetic feedback regulation in the dynamics of the epithelial-mesenchymal transition (EMT)
Arabidopsis ATXR2 deposits H3K36me3 at the promoters of LBD genes to facilitate cellular dedifferentiation
Modulation of Mitochondrial DNA Copy Number to Induce Hepatocytic Differentiation of Human Amniotic Epithelial Cells
Exploring the mechanisms of cell reprogramming and transdifferentiation via intercellular communication
Integrating cardiomyocytes from human pluripotent stem cells in safety pharmacology: has the time come?
Osteogenesis depends on commissioning of a network of stem cell transcription factors that act as repressors of adipogenesis.
A mathematical model exhibiting the effect of DNA methylation on the stability boundary in cell-fate networks.
Comparison of multi-lineage differentiation of hiPSCs reveals novel miRNAs that regulate lineage specification
Hematopoietic stem cells from induced pluripotent stem cells - considering the role of microRNA as a cell differentiation regulator
Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription
Clinical-pathological correlations of BAV and the attendant thoracic aortopathies. Part 2: Pluridisciplinary perspective on their genetic and molecular origins
Cell Fate Conversion By mRNA
mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.