Pluripotency of bovine embryonic cell line derived from precompacting embryos

Cloning
M MitalipovaN L First

Abstract

We report herein the establishment of three bovine pluripotent embryonic cell lines derived from 8-16-cell precompacting embryos. Two cell lines were cultured for 10 passages and underwent spontaneous differentiation. One cell line (Z2) has been cultured continuously for over 3 years and has remained undifferentiated. These cells express cell surface markers that have been used routinely to characterize embryonic stem (ES) and embryonic germ (EG) cells in other species such as stage-specific embryonic antigens SSEA-1, SSEA-3, and SSEA-4, and c-Kit receptor. In the absence of a feeder layer, these cells differentiated into a variety of cell types and formed embryoid bodies (EBs). When cultured for an extended period of time, EBs differentiated into derivatives of three EG layers - mesoderm, ectoderm, and endoderm - which were characterized by detection of specific cell surface markers. Our results indicate that the Z2 cell line is pluripotent and resembles an ES cell line. To our knowledge, this is the first bovine embryonic cell line that has remained pluripotent in culture for more than 150 passages.

References

Jul 1, 1988·Differentiation; Research in Biological Diversity·B A FendersonS Hakomori
May 1, 1988·Developmental Biology·T DoetschmanN Maeda
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A ThomsonJ P Hearn
Jan 1, 1994·Reproduction, Fertility, and Development·N L FirstM J Kent-First
Jan 1, 1994·Reproduction, Fertility, and Development·M B Wheeler
Apr 1, 1995·Molecular Reproduction and Development·A E Van Stekelenburg-HamersC L Mummery
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Sims, N L First
Sep 1, 1993·Biology of Reproduction·C F RosenkransN L First
Aug 1, 1996·Biology of Reproduction·J A ThomsonJ P Hearn
May 29, 1998·Biology of Reproduction·J A PiedrahitaT Ott
Nov 6, 1998·Science·J A ThomsonJ M Jones
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·M J ShamblottJ D Gearhart
Aug 7, 1999·Veterinary Immunology and Immunopathology·H HikonoM Sakurai
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·T WakayamaP Mombaerts
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso

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Citations

Sep 27, 2003·Trends in Biotechnology·David N WellsGötz Laible
May 30, 2002·Journal of Anatomy·Michal Amit, Joseph Itskovitz-Eldor
Jan 21, 2009·Reproduction, Fertility, and Development·Björn Oback
Apr 1, 2010·Reproduction, Fertility, and Development·Esben OestrupPoul Hyttel
Oct 10, 2012·Reproduction, Fertility, and Development·Ruchi SharmaPrabhat Palta
Dec 19, 2012·Reproduction, Fertility, and Development·Monika Nowak-Imialek, Heiner Niemann
Aug 4, 2010·Cellular Reprogramming·Guochun GongXiuchun Cindy Tian
Aug 4, 2010·Cellular Reprogramming·Ivan VassilievMark B Nottle
Oct 13, 2010·Cellular Reprogramming·Maryam PashaiaslPaul J Verma
Jul 15, 2009·Cloning and Stem Cells·Disha Pant, Carol L Keefer
Mar 31, 2009·Stem Cells and Development·Jared T WilcoxDean H Betts
Apr 13, 2010·Stem Cells and Development·Franklin D WestSteven L Stice
Jan 4, 2013·Stem Cells and Development·Vinod VermaBjörn Oback
Mar 4, 2005·Biology of Reproduction·Li WangX Cindy Tian
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·I A Grivennikov
Jan 17, 2008·The Journal of Reproduction and Development·Irena VackovaFederica Lopes
Jan 7, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Joëlle A DesmaraisLawrence C Smith
Jul 10, 2008·Stem Cell Reviews·Neil C Talbot, Le Ann Blomberg
Dec 2, 2009·Stem Cell Reviews and Reports·Bhanu Prakash V L TeluguR Michael Roberts
Feb 3, 2005·Molecular Reproduction and Development·Morten VejlstedPoul Maddox-Hyttel
Apr 3, 2009·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Shanbo CaoLin Liu
Feb 10, 2007·Molecular Reproduction and Development·Hathaitip SritanaudomchaiThanit Kusamran
Nov 8, 2007·Reproduction in Domestic Animals = Zuchthygiene·S-K ChiangJ-C Ju
Sep 23, 2008·Reproduction in Domestic Animals = Zuchthygiene·M MuñozE Gómez
Jan 16, 2008·Molecular Reproduction and Development·Xianfeng YuIlkeun Kong
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·R Alberio, A R Perez
Sep 25, 2012·Reproduction in Domestic Animals = Zuchthygiene·L A Blomberg, B P V L Telugu
Mar 10, 2011·Molecular Reproduction and Development·E BehboodiI Dobrinski
Aug 29, 2003·Biochemical and Biophysical Research Communications·Shigeo SaitoKazunari K Yokoyama
Oct 6, 2005·Molecular Reproduction and Development·M DattenaP Cappai
May 15, 2012·Theriogenology·Luis Fernando Malaver-OrtegaPaul J Verma

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