PMID: 7538677May 9, 1995Paper

Pluripotent hematopoietic stem cells contain high levels of mRNA for c-kit, GATA-2, p45 NF-E2, and c-myb and low levels or no mRNA for c-fms and the receptors for granulocyte colony-stimulating factor and interleukins 5 and 7

Proceedings of the National Academy of Sciences of the United States of America
D OrlicD M Bodine

Abstract

Pluripotent hematopoietic stem cells (PHSCs) were highly enriched from mouse bone marrow by counterflow centrifugal elutriation, lineage subtraction, and fluorescence-activated cell sorting based on high c-kit receptor expression (c-kitBR). We used reverse transcriptase polymerase chain reaction to assay the c-kitBR subset and the subsets expressing low (c-kitDULL) and no (c-kitNEG) c-kit receptor for expression of mRNA encoding hematopoietic growth factor receptors and transcription factors. The c-kitBR cells had approximately 3.5-fold more c-kit mRNA than unfractionated bone marrow cells. The c-kitDULL cells had 47-58% of the c-kit mRNA found in c-kitBR cells and the c-kitNEG cells had 4-9% of the c-kit mRNA present in c-kitBR cells. By comparing mRNA levels in c-kitBR cells (enriched for PHSCs) with those of unfractionated bone marrow, we demonstrated that c-kitBR cells contained low or undetectable levels of mRNA for c-fms, granulocyte colony-stimulating factor receptor, interleukin 5 receptor (IL-5R), and IL-7R. These same cells had moderate levels of mRNA for erythropoietin receptor, IL-3R subunits IL-3R alpha (SUT-1), AIC-2A, and AIC-2B, IL-6R and its partner gp-130, and the transcription factor GATA-1 and high levels of...Continue Reading

References

Jul 1, 1988·Science·G J SpangrudeI L Weissman
Oct 1, 1966·Experimental Cell Research·D H Pluznik, L Sachs
Jun 1, 1984·The Journal of Experimental Medicine·J W VisserA M de Leeuw

❮ Previous
Next ❯

Citations

May 20, 1998·Biotherapy·A R Migliaccio, G Migliaccio
Feb 5, 2008·Science in China. Series C, Life Sciences·HuiJie JiangNakano Toru
Jan 9, 2001·Experimental Hematology·P IkonomiC T Noguchi
Jul 5, 2003·Experimental Hematology·Hong GuoRobort Chunhua Zhao
Mar 18, 2000·Experimental Hematology·M V ZinovyevaA V Belyavsky
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J Bauer
Jan 17, 2013·The Journal of Biological Chemistry·Erin Eun-Young AhnDong-Er Zhang
Oct 7, 2004·The Journal of Experimental Medicine·Kam-Wing LingElaine Dzierzak
Jan 11, 2012·The Journal of Experimental Medicine·Kai B KaufmannHeike L Pahl
Dec 4, 2013·The Journal of Experimental Medicine·Emma de PaterElaine Dzierzak
Jun 30, 1998·APMIS. Supplementum·J Olweus
Jun 1, 1997·Immunological Reviews·H L AguilaI L Weissman
Jul 21, 1998·Annals of the New York Academy of Sciences·D M BodineD Orlic
Jun 18, 1999·Annals of the New York Academy of Sciences·D OrlicD M Bodine
Sep 19, 2006·Molecular and Cellular Biology·Jeffrey A GrassEmery H Bresnick
Aug 2, 2005·Molecular and Cellular Biology·Maki Kobayashi-OsakiMasayuki Yamamoto
Jul 3, 2010·American Journal of Respiratory and Critical Care Medicine·Georgios T StathopoulosTimothy S Blackwell
Jun 26, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J ZychA Correa
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Ye Zhou, Scott A Ness
Jun 30, 2000·Biochemical and Biophysical Research Communications·R WieserC Fonatsch
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Saumen PalEmery H Bresnick
Sep 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael J NemethDavid M Bodine
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G L Evans, R A Morgan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.