Plus- and minus-end directed microtubule motors bind simultaneously to herpes simplex virus capsids using different inner tegument structures.

PLoS Pathogens
Kerstin RadtkeBeate Sodeik

Abstract

Many viruses depend on host microtubule motors to reach their destined intracellular location. Viral particles of neurotropic alphaherpesviruses such as herpes simplex virus 1 (HSV1) show bidirectional transport towards the cell center as well as the periphery, indicating that they utilize microtubule motors of opposing directionality. To understand the mechanisms of specific motor recruitment, it is necessary to characterize the molecular composition of such motile viral structures. We have generated HSV1 capsids with different surface features without impairing their overall architecture, and show that in a mammalian cell-free system the microtubule motors dynein and kinesin-1 and the dynein cofactor dynactin could interact directly with capsids independent of other host factors. The capsid composition and surface was analyzed with respect to 23 structural proteins that are potentially exposed to the cytosol during virus assembly or cell entry. Many of these proteins belong to the tegument, the hallmark of all herpesviruses located between the capsid and the viral envelope. Using immunoblots, quantitative mass spectrometry and quantitative immunoelectron microscopy, we show that capsids exposing inner tegument proteins such a...Continue Reading

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Citations

Oct 9, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael J CastleJohn H Wolfe
Jun 26, 2012·Annual Review of Microbiology·Gregory Smith
Feb 19, 2013·Cell Host & Microbe·Sofia V ZaichickGregory A Smith
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Feb 24, 2012·PLoS Pathogens·L W Enquist
Feb 15, 2013·Journal of Virology·Daniel HenaffRoger Lippé
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Mar 31, 2015·Nature Communications·Viacheslav MalikovMojgan H Naghavi

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Methods Mentioned

BETA
electron microscopy
FCS
light scattering
fluorescence microscopy

Software Mentioned

AmaDeuS

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