PMID: 9531971Apr 9, 1998Paper

PMA alters folate receptor distribution in the plasma membrane and increases the rate of 5-methyltetrahydrofolate delivery in mature MA104 cells

Biochimica Et Biophysica Acta
C M LewisBarton A Kamen

Abstract

MA104 cells (a monkey kidney cell line) can internalize 5-methyltetrahydrofolate via a receptor mediated process termed potocytosis. Uptake is initiated by binding to an external folate receptor which cycles to an internal, but membrane bound compartment. These two pools can be measured by determining the amount of [3H]ligand removed by an acid-saline wash, i.e. acid labile and acid resistant pools. When assayed in confluent nonmitotic cells, 2/3 of the folate receptor pool is located in an internal (acid resistant) compartment, but phorbol 12-myristate 13-acetate (PMA) causes a shift such that 65-75% of the receptor pool resides on the surface of the plasma membrane. This new steady state is likely the result of an increased rate of receptor movement. In addition, PMA increases the rate of 5-methyl[3H]tetrahydrofolate delivery to the cytoplasm 1.8 fold. Using known inhibitors of potocytosis, we were able to show that the increased rate of delivery is receptor mediated. Comparison of the time courses of the PMA effects on folate receptor redistribution assessed by membrane binding of [3H]folic acid and 5-methyl[3H]tetrahydrofolate delivery to the cytoplasm suggests that PMA may be activating more than one protein kinase C indep...Continue Reading

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Citations

Mar 3, 2007·Cancer Chemotherapy and Pharmacology·Stewart P JohnsonHenry S Friedman
Apr 20, 2004·Advanced Drug Delivery Reviews·Shefali Sabharanjak, Satyajit Mayor
Nov 16, 2004·International Journal of Pharmaceutics·Liangke ZhangYi Lu
Dec 19, 2007·Journal of Neurochemistry·Jan B WollackBarton Kamen

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