PML3 Orchestrates the Nuclear Dynamics and Function of TIP60.

The Journal of Biological Chemistry
Quan WuXuebiao Yao

Abstract

The promyelocytic leukemia (PML) protein is a major component to govern the PML nuclear body (NB) assembly and function. Although it is well defined that PML NB is a site recruiting sumoylated proteins, the mechanism by which PML protein regulates the process remains unclear. Here we show that PML3, a specific PML isoform, interacts with and recruits TIP60 to PML NBs. Our biochemical characterization demonstrates that PML3 physically interacts with TIP60 via its N-terminal 364 amino acids. Importantly, this portion of TIP60 is sufficient to target to the PML NBs, suggesting that PML3-TIP60 interaction is sufficient for targeting TIP60 to the NBs. The PML3-TIP60 interaction is specific, since the region of TIP60 binding is not conserved in other PML isoforms. The physical interaction between PML3 and TIP60 protects TIP60 from Mdm2-mediated degradation, suggesting that PML3 competes with MDM2 for binding to TIP60. Fluorescence recovery after photobleaching analysis indicates that the PML3-TIP60 interaction modulates the nuclear body distribution and mobility of TIP60. Conversely, the distribution and mobility of TIP60 are perturbed in PML3-deficient cells, accompanied by aberrations in DNA damage-repairing response. Thus, PML3 or...Continue Reading

References

Mar 1, 1991·The Journal of Cell Biology·C A Ascoli, G G Maul
Oct 1, 1994·Molecular and Cellular Biology·Z M MuK S Chang
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·K L BordenP S Freemont
Apr 16, 1998·Genes & Development·K Struhl
Jul 31, 1998·American Journal of Human Genetics·M HodgesP S Freemont
Oct 3, 1998·The Journal of Biological Chemistry·T KamitaniE T Yeh
Oct 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M H Kuo, C D Allis
Jun 11, 1999·The Journal of Biological Chemistry·M E BradyC N Robson
May 12, 2000·Journal of Structural Biology·G G MaulA M Ishov
May 12, 2000·Nature Cell Biology·S ZhongP P Pandolfi
Nov 18, 2000·The EMBO Journal·V FogalG Del Sal
Nov 13, 2001·Oncogene·K JensenP S Freemont
Dec 7, 2002·The Journal of Biological Chemistry·Claudie LemercierSaadi Khochbin
Jun 18, 2003·The Journal of Biological Chemistry·Igal Louria-HayonYgal Haupt
Aug 23, 2003·Cell Death and Differentiation·T G Hofmann, H Will
Sep 26, 2003·The Journal of Biological Chemistry·Hongyan ZhuCarl G Maki
Aug 18, 2004·The Journal of Biological Chemistry·Gaëlle LegubeDidier Trouche
Sep 8, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Graham Dellaire, David P Bazett-Jones
Dec 2, 2004·Molecular and Cellular Biology·Jagruti H PatelSteven B McMahon
Mar 8, 2005·Molecular Cell·Zhi-Xiang XuKun-Sang Chang
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yingli SunBrendan D Price
Sep 22, 2005·Nature Reviews. Microbiology·Sébastien NisoleAli Saïb
May 16, 2006·The International Journal of Biochemistry & Cell Biology·Vasileia SapountziCraig N Robson
Aug 15, 2006·Trends in Cell Biology·Massimo SquatritoBruno Amati
Nov 4, 2006·Molecular Cell·Tian Huai ShenPier Paolo Pandolfi
May 28, 2008·Cell Research·P SalomoniT Rich

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Citations

Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Susumu RokudaiCarol Prives
Jan 26, 2013·International Journal of Molecular Sciences·Weiwei LaiYongguang Tao
Jun 15, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kotaro KoiwaiOsamu Koiwai
Jan 13, 2018·Journal of Virology·Hironori NishitsujiKunitada Shimotohno
Oct 18, 2020·Nucleic Acids Research·Armelle CorpetPatrick Lomonte
Dec 29, 2020·Virus Research·Samuel HofmannSabrina Schreiner

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