pmp1+, a suppressor of calcineurin deficiency, encodes a novel MAP kinase phosphatase in fission yeast

The EMBO Journal
Reiko SugiuraTakayoshi Kuno

Abstract

Calcineurin is a highly conserved and ubiquitously expressed Ca2+- and calmodulin-dependent protein phosphatase. The in vivo role of calcineurin, however, is not fully understood. Here, we show that disruption of the calcineurin gene (ppb1(+)) in fission yeast results in a drastic chloride ion (Cl-)-sensitive growth defect and that a high copy number of a novel gene pmp1(+) suppresses this defect. pmp1(+) encodes a phosphatase, most closely related to mitogen-activated protein (MAP) kinase phosphatases of the CL100/MKP-1 family. Pmp1 and calcineurin share an essential function in Cl- homeostasis, cytokinesis and cell viability. Pmp1 phosphatase dephosphorylates Pmk1, the third MAP kinase in fission yeast, in vitro and in vivo, and is bound to Pmk1 in vivo, strongly suggesting that Pmp1 negatively regulates Pmk1 MAP kinase by direct dephosphorylation. Consistently, the deletion of pmk1(+) suppresses the Cl--sensitive growth defect of ppb1 null. Thus, calcineurin and the Pmk1 MAP kinase pathway may play antagonistic functional roles in the Cl- homeostasis.

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Citations

Sep 15, 2006·Molecular Genetics and Genomics : MGG·Nimisha SharmaJürg Bähler
Jun 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Reiko SugiuraTakayoshi Kuno
Oct 2, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yabin LuTakayoshi Kuno
Jun 12, 2012·The Journal of Biological Chemistry·Laura Sánchez-MirJosé Cansado
Sep 21, 2007·Molecular Biology of the Cell·Hirofumi TakadaReiko Sugiura
Jul 25, 2009·Molecular Biology of the Cell·Andrés NúñezJosé Cansado
Mar 6, 2003·Genome Research·Anabelle DecottigniesPaul Nurse
Mar 28, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Reiko Sugiura
Mar 26, 2003·The Journal of Cell Biology·Ana BerlinFred Chang
Oct 24, 1998·The Journal of Cell Biology·J Demeter, S Sazer
Feb 20, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Thi Thuy Trang NguyenEe Sin Chen
Jul 22, 2011·Journal of Signal Transduction·Reiko SugiuraAyako Kita
Mar 26, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kentaro NakanoIssei Mabuchi
Jan 16, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takuya MaedaTakayoshi Kuno
Apr 18, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yuichiro HanyuMitsuhiro Yanagida
Sep 17, 2008·Molecular Microbiology·M Antonia Villar-TajaduraPilar Pérez
Jul 14, 2010·Molecular Microbiology·Angela CadouXavier Le Goff
Jun 21, 2011·Journal of Cellular Physiology·Jarrett T WhelanMyon-Hee Lee
Dec 23, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mari HigaReiko Sugiura
Feb 6, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akira DoiReiko Sugiura
Feb 6, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akira DoiReiko Sugiura
Jan 13, 2015·Biochemical and Biophysical Research Communications·Ayako KitaReiko Sugiura

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