Pmr, a histone-like protein H1 (H-NS) family protein encoded by the IncP-7 plasmid pCAR1, is a key global regulator that alters host function.

Journal of Bacteriology
Choong-Soo YunHideaki Nojiri

Abstract

Histone-like protein H1 (H-NS) family proteins are nucleoid-associated proteins (NAPs) conserved among many bacterial species. The IncP-7 plasmid pCAR1 is transmissible among various Pseudomonas strains and carries a gene encoding the H-NS family protein, Pmr. Pseudomonas putida KT2440 is a host of pCAR1, which harbors five genes encoding the H-NS family proteins PP_1366 (TurA), PP_3765 (TurB), PP_0017 (TurC), PP_3693 (TurD), and PP_2947 (TurE). Quantitative reverse transcription-PCR (qRT-PCR) demonstrated that the presence of pCAR1 does not affect the transcription of these five genes and that only pmr, turA, and turB were primarily transcribed in KT2440(pCAR1). In vitro pull-down assays revealed that Pmr strongly interacted with itself and with TurA, TurB, and TurE. Transcriptome comparisons of the pmr disruptant, KT2440, and KT2440(pCAR1) strains indicated that pmr disruption had greater effects on the host transcriptome than did pCAR1 carriage. The transcriptional levels of some genes that increased with pCAR1 carriage, such as the mexEF-oprN efflux pump genes and parI, reverted with pmr disruption to levels in pCAR1-free KT2440. Transcriptional levels of putative horizontally acquired host genes were not altered by pCAR1 c...Continue Reading

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Citations

Feb 22, 2012·Applied and Environmental Microbiology·Kevin S LangTimothy J Johnson
Jan 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hideaki Nojiri
Feb 26, 2011·International Journal of Evolutionary Biology·Hiromi Nishida, Choong-Soo Yun
Feb 26, 2011·International Journal of Evolutionary Biology·Toshiharu TakedaHideaki Nojiri
Jan 9, 2016·Environmental Microbiology Reports·Seunguk LeeHideaki Nojiri
Jun 4, 2014·Environmental Microbiology·Yurika TakahashiHideaki Nojiri
Jul 4, 2012·Mobile Genetic Elements·Timothy J Johnson, Kevin S Lang
Oct 23, 2012·Current Opinion in Biotechnology·Hideaki Nojiri
Aug 6, 2016·Plasmid·Kevin S Lang, Timothy J Johnson
Jan 13, 2017·Bioscience, Biotechnology, and Biochemistry·Masaki Shintani
Feb 15, 2015·Applied and Environmental Microbiology·Chiho Suzuki-MinakuchiHideaki Nojiri
Nov 30, 2019·Environmental Microbiology·Ludovic Vial, Florence Hommais
Dec 12, 2020·Computational and Structural Biotechnology Journal·Jian Jiao, Chang-Fu Tian
Jun 25, 2015·Microbiology Spectrum·Ana SeguraJuan Luis Ramos
Mar 21, 2021·Applied and Environmental Microbiology·Joydeep ChakrabortyHideaki Nojiri
Nov 30, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kathryn BillaneMichael A Brockhurst

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