Pneumococcal 6-phosphogluconate-dehydrogenase, a putative adhesin, induces protective immune response in mice.

Clinical and Experimental Immunology
D DanielyY Mizrachi Nebenzahl

Abstract

For most bacteria, adherence to human cells is achieved by bacterial lectins binding to mammalian surface glyconjugates. 6-Phosphogluconate dehydrogenase (6PGD) was identified by us as one of Streptococcus pneumoniae cell wall lectin proteins, which elicits an age-dependent immune response in humans. This study assesses the role of 6PGD in S. pneumoniae pathogenesis as an adhesin and its ability to elicit a protective immune response in mice. Recombinant 6PGD (r6PGD) was cloned from S. pneumoniae serotype 3 (strain WU2). r6PGD interference in adhesion of three genetically unrelated unencapsulated pneumococcal strains (3.8, 14.8 and R6) and two genetically unrelated encapsulated pneumococcal strains (WU2 and D39) to A549 type II lung carcinoma cell was tested. BALB/c mice were immunized with r6PGD and boosted after 3 weeks. Immunized mice were challenged intranasally with a lethal dose of S. pneumoniae. r6PGD inhibited 90% and 80% of pneumococcal adhesion to the A549 cells of three unencapsulated S. pneumoniae strains and two encapsulated S. pneumoniae strains, respectively, in a concentration-dependent manner (P < 0.05). Antibodies to r6PGD produced in mice significantly inhibited bacterial adhesion to A549 cell (P < 0.05). Imm...Continue Reading

References

Jan 15, 1976·International Journal of Cancer. Journal International Du Cancer·M LieberG Todaro
Apr 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D M Musher
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H C KrivanV Ginsburg
Feb 1, 1981·Infection and Immunity·J L SiegelA S Bleiweis
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·K AsanoJ S Stamler
Jun 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D A WatsonJ Verhoef
Sep 1, 1993·Molecular Microbiology·B J PearceH R Masure
Sep 1, 1996·Infection and Immunity·U M TalbotJ C Paton
Sep 18, 1997·The Journal of Infectious Diseases·I Idänpään-HeikkiläE Tuomanen
Nov 30, 2000·Biochemical and Biophysical Research Communications·S S D'CostaM D Boyle
Feb 22, 2001·The Journal of Infectious Diseases·D M MusherR E Baughn
Feb 21, 2002·Infection and Immunity·Martin J G HughesJoseph D Santangelo
Mar 6, 2002·Clinical and Experimental Immunology·S LifshitzY Mizrachi Nebenzahl
Mar 27, 2004·FEMS Microbiology Letters·Y Mizrachi NebenzahlR Dagan
Apr 23, 2004·Infection and Immunity·Josée HamelBernard R Brodeur
Jun 25, 2004·The Journal of Biological Chemistry·Bastian OpitzStefan Hippenstiel
Jul 17, 2004·Molecular Microbiology·Lauren J McAllisterJames C Paton
Nov 18, 2004·Biochimica Et Biophysica Acta·Hung Ton-ThatOlaf Schneewind
Apr 6, 2005·Research in Microbiology·Shaynoor DramsiHélène Bierne
Jul 26, 2005·Infection and Immunity·Sven HammerschmidtManfred Rohde
Apr 23, 2008·Current Protocols in Bioinformatics·Laura ElnitskiWebb Miller

❮ Previous
Next ❯

Citations

Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Angela H NobbsHoward F Jenkinson
Aug 22, 2012·Canadian Journal of Microbiology·Helton Fernandes AraújoSophie Yvette Leclercq
Jan 12, 2013·Current Protein & Peptide Science·Ruth Dennehy, Siobhan McClean
Feb 2, 2013·Applied Microbiology and Biotechnology·Jian WangShuo Wang
Jul 5, 2012·Molecular Oral Microbiology·I Pérez-DoradoJ A Hermoso
Jan 25, 2011·Microbial Biotechnology·Monica MoschioniMichèle A Barocchi
Nov 18, 2014·Biochemical Society Transactions·Brian Henderson
Jun 26, 2015·PloS One·Vaishak Amblee, Constance J Jeffery
Apr 17, 2016·Environmental Microbiology Reports·Juan J QueredaM Graciela Pucciarelli
Mar 1, 2008·Proteomics. Clinical Applications·Jason N ColeMark J Walker
Sep 24, 2013·Expert Review of Vaccines·Christian Schulz, Sven Hammerschmidt
Dec 7, 2016·Molecular Medicine Reports·Sara NovickYaffa Mizrachi Nebenzahl
Jun 12, 2020·The Journal of Microbiology·Kesavan DineshkumarHuaxi Xu
Aug 27, 2011·Mycopathologia·Santosh Kumar UpadhyayTaruna Madan
Dec 21, 2019·Microbiology Spectrum·D E BrilesM J Crain
Jun 25, 2021·Journal of Molecular Recognition : JMR·Gideon Fleminger, Avraham Dayan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.