Pneumococcal whole-cell vaccine: optimization of cell growth of unencapsulated Streptococcus pneumoniae in bioreactor using animal-free medium.

Journal of Industrial Microbiology & Biotechnology
Celia LibermanViviane M Gonçalves

Abstract

The high cost of the available pneumococcal conjugated vaccines has been an obstacle in implementing vaccination programs for children in developing countries. As an alternative, Malley et al. proposed a vaccine consisting of inactivated whole-cells of unencapsulated S. pneumoniae, which provides serotype-independent protection and involves lower production costs. Although the pneumococcus has been extensively studied, little research has focused on its large-scale culture, thus implying a lack of knowledge of process parameters, which in turn are essential for its successful industrial production. The strain Rx1Al- eryR was originally cultured in Todd-Hewitt medium (THY), which is normally used for pneumococcus isolation, but is unsuitable for human vaccine preparations. The purposes of this study were to compare the strains Rx1Al- eryR and kanR, develop a new medium, and generate new data parameters for scaling-up the process. In static flasks, cell densities were higher for eryR than kanR. In contrast, the optical density (OD) of the former decreased immediately after reaching the stationary phase, and the OD of the latter remained stable. The strain Rx1Al- kanR was cultivated in bioreactors with medium based on either acid-...Continue Reading

References

Jul 1, 1994·Journal of Clinical Microbiology·C P CartwrightV J Gill
Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Richard MalleyMarc Lipsitch

❮ Previous
Next ❯

Citations

Aug 25, 2015·Applied Microbiology and Biotechnology·Bruno Vitorio MarthosViviane Maimoni Gonçalves
Apr 10, 2013·Computer Methods and Programs in Biomedicine·M C NicolettiR C Giordano
Aug 23, 2018·Journal of Applied Microbiology·M MohammadzadehS Mamishi
Aug 15, 2015·Clinical and Experimental Vaccine Research·Ho Seong Seo
Oct 19, 2019·Vaccines·Victor MoraisNorma Suarez
Feb 19, 2020·Biotechnology and Bioengineering·Ivana B CamposViviane M Gonçalves

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.