Pneumocystis cell wall beta-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells.

Respiratory Research
Eva M CarmonaAndrew H Limper

Abstract

Respiratory failure secondary to alveolar inflammation during Pneumocystis pneumonia is a major cause of death in immunocompromised patients. Neutrophil infiltration in the lung of patients with Pneumocystis infection predicts severity of the infection and death. Several previous studies indicate that airway epithelial cells release the neutrophil chemoattractant proteins, MIP-2 (rodents) and IL-8 (humans), in response to Pneumocystis and purified Pneumocystis cell wall beta-glucans (PCBG) through the NF-kappaB-dependent pathway. However, little is known about the molecular mechanisms that are involved in the activation of airway epithelium cells by PCBG resulting in the secretion of IL-8. To address this, we have studied the activation of different calcium-dependent mitogen-activated protein kinases (MAPKs) in 1HAEo- cells, a human airway epithelial cell line. Our data provide evidence that PCBG induces phosphorylation of the MAPKs, ERK, and p38, the activation of NF-kappaB and the subsequently secretion of IL-8 in a calcium-dependent manner. Further, we evaluated the role of glycosphingolipids as possible receptors for beta-glucans in human airway epithelial cells. Preincubation of the cells with D-threo-1-phenyl-2-decanoylam...Continue Reading

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Citations

Jun 26, 2013·Trends in Microbiology·Scott G Filler
Jul 10, 2013·Infection and Immunity·David M RicksJay K Kolls
Jun 7, 2011·American Journal of Respiratory Cell and Molecular Biology·Wendy A NeveuMercedes Rincon
Feb 22, 2012·American Journal of Respiratory Cell and Molecular Biology·Eva M CarmonaAndrew H Limper
Jun 14, 2014·European Journal of Cell Biology·Kana LinErqing Wei
Jun 22, 2016·The Journal of Infectious Diseases·Geetha KuttyJoseph A Kovacs
Dec 3, 2016·Medical Mycology Journal·Yinggai SongRuoyu Li
Jun 15, 2018·Clinical Microbiology Reviews·Liang MaJoseph A Kovacs
Oct 18, 2018·Journal of Medical Microbiology·Theodore J KottomAndrew H Limper
Feb 6, 2020·Cellular Microbiology·Yueqin LiuJoseph A Kovacs
Sep 10, 2011·Interdisciplinary Perspectives on Infectious Diseases·Jing WangFrancis Gigliotti
Dec 13, 2016·Current Allergy and Asthma Reports·Zhonghua ZhangGurjit K Khurana Hershey
Sep 9, 2017·American Journal of Respiratory Cell and Molecular Biology·Theodore J KottomAndrew H Limper
Mar 25, 2017·International Journal of Molecular Sciences·Chang-Ching YehPeng-Hui Wang
Feb 19, 2020·International Archives of Allergy and Immunology·Lorraine Tsitsi PfavayiFrancisca Mutapi

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Methods Mentioned

BETA
ELISA
fluorescence imaging
protein assay
transfection
targeted mutations

Software Mentioned

GraphPad
GraphPad Prism
ImageJ

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