Pneumoperitoneum augmented tumor invasiveness is abolished by matrix metalloproteinase blockade

Surgical Endoscopy
P F RidgwayA Darzi

Abstract

Certain surgical strategies, including Helium (He) and carbon dioxide (CO2) insufflation in laparoscopy, have been shown to induce a hypoxic environment. This may have a significant effect on the invasive capacity of tumor cells and may be a factor in the incidence of port-site metastases seen in patients following laparoscopic resection for malignancy. A colon adenocarcinoma cell line (SW1222) was exposed to an in vitro pneumoperitoneum of CO2 or He at 3 mmHg or left in normal growth conditions (control). After a 4-hour exposure to an in vitro pneumoperitoneum, the ability of the cells to invade through 8.0-microm Transwell filters coated with Matrigel was analyzed by colorimetric MTS assay and by direct staining of the filters. The effect of the addition of a known blocker of matrix metalloproteinases (MMPs), 1,10-phenanthroline (1,10-P), was investigated. Cells exposed to an in vitro pneumoperitoneum demonstrate significantly increased invasive capacity compared to the control set, without loss of viability (He vs control, p <0.001; CO2 vs control, p <0.001). This augmented capacity is abolished by the addition of 1,10-P (p <0.01). Exposure of a colonic adenocarcinoma cell line to either a CO2 or He pneumoperitoneum causes a...Continue Reading

Citations

Nov 6, 2012·Pediatric Surgery International·Angela Simona MontaltoCarmelo Romeo
Dec 3, 2008·Clinical and Experimental Medicine·Ying-Xue HaoBo Tang
Aug 17, 2006·Clinical & Experimental Metastasis·P A ParaskevaA W Darzi
Oct 8, 2003·Gynecologic Oncology·Pedro T RamirezCharles Levenback
Jan 9, 2003·Journal of the American College of Surgeons·Steven D Wexner, Dana R Sands
Mar 18, 2005·Journal of Laparoendoscopic & Advanced Surgical Techniques. Part a·Chandrakanth Are, Mark A Talamini
Apr 16, 2005·The Cancer Journal·James R OuelletteAlan T Lefor
May 18, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·P A ParaskevaA W Darzi
Sep 17, 2002·Journal of the American College of Surgeons·Paul ZiprinAra W Darzi
Dec 18, 2021·Nature Reviews. Disease Primers·Delia Cortés-GuiralManish Chand

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