Pneumothorax-associated pleural eosinophilia is tumour necrosis factor-alpha-dependent and attenuated by steroids

Respirology : Official Journal of the Asian Pacific Society of Respirology
Ioannis KalomenidisCharis Roussos

Abstract

The pathogenesis and the optimal treatment of eosinophilic pleural effusions are unknown. We aimed to examine whether pneumothorax-associated pleural eosinophilia in mice is dependent on tumour necrosis factor (TNF)-alpha, and whether it is affected by systemic administration of corticosteroids. Mice were injected intrapleurally with 0.4 mL air to create pneumothoraces. Animals were sacrificed 24 or 48 h later, and pleural lavage (PL) was performed. In the first experiment, comparisons were made between wild-type and TNF-alpha knockout mice with pneumothorax. In the second experiment, wild-type mice were injected intraperitoneally with different doses of dexamethasone (0, 0.25, 0.5 and 1 mg/kg), 5 min before and 24 h after the induction of pneumothorax. After induction of a pneumothorax, TNF-alpha knockout mice had significantly fewer total number of cells (P = 0.004), mononuclear cells (P = 0.01), neutrophils (P = 0.017) and eosinophils (P = 0.002) in their PL compared with wild-type animals. TNF-alpha was detected in the PL of most of the control mice but not in TNF-alpha knockouts. Dexamethasone induced a significant, dose-dependent reduction of PL total cells (P < 0.001), eosinophils (P < 0.001), mononuclear cells (P = 0.00...Continue Reading

References

Dec 15, 1993·Annals of Internal Medicine·D T BoumpasJ E Balow
Mar 22, 2000·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·H D FladM Ernst
Jun 13, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A P Sampson
Dec 4, 2001·European Journal of Pharmacology·X ZhangH Kankaanranta
Jun 14, 2003·Current Opinion in Pulmonary Medicine·Ioannis Kalomenidis, Richard W Light
Sep 17, 2003·Apoptosis : an International Journal on Programmed Cell Death·A DruilheM Pretolani
Feb 26, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Enas A HamedAzza Mahmoud
Jun 29, 2004·The European Respiratory Journal·A De SmedtM Noppen
Jun 29, 2004·Current Opinion in Pulmonary Medicine·Ioannis Kalomenidis, Richard W Light
Feb 3, 2006·The European Respiratory Journal·P J Barnes
Oct 13, 2006·Respiratory Research·Srirupa MukhopadhyayTapan K Mukherjee

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Citations

Apr 9, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Martin R J Kolb, Ian Yang

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