PO-CALC: a novel tool to correct common inconsistencies in the measurement of phenoloxidase activity

Journal of Insect Physiology
Philip KohlmeierJoël Meunier

Abstract

A broad range of physiological and evolutionarily studies requires standard and robust methods to assess the strength and activity of an individual's immune defense. In insects, this goal is generally reached by spectrophotometrically measuring (pro-) phenoloxidase activity, an enzymatic and non-specific process activated after wounding and parasite infections. However, the literature surprisingly lacks a standard method to calculate these values from spectrophotometer data and thus to be able to compare results across studies. In this study, we demonstrated that nine methods commonly used to extract phenoloxidase activities (1) provide inconsistent results when tested on the same data sets, at least partly due to their specific sensitivity to the noise regularly present in enzymatic reaction curves. To circumvent this issue, we then (2) developed a novel, free and simple R-based program called PO-CALC and (3) demonstrated the robustness of its calculations for the different types of noises. Overall, we show that PO-CALC corrects overlooked though important inconsistencies in the measurement of phenoloxidase activities, and claim that its broad use would increase the significance and general validity of studies on invertebrate ...Continue Reading

References

Sep 3, 2002·Proceedings. Biological Sciences·Markus J RantalaJukka Suhonen
Feb 28, 2004·Physiological and Biochemical Zoology : PBZ·Patrick T Mucklow, Dieter Ebert
Jun 18, 2004·Immunological Reviews·Lage Cerenius, Kenneth Söderhäll
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ioannis EleftherianosStuart E Reynolds
Sep 25, 2008·The American Naturalist·B Kraus, R E Page
May 24, 2013·Proceedings. Biological Sciences·I M DubovskiyT M Butt
May 31, 2013·Biology Letters·Kathryn B McNamaraLeigh W Simmons
Jun 4, 2013·Journal of Insect Physiology·Angelica V PiñeraKathleen A Killian
Mar 19, 2014·Journal of Insect Physiology·Stephanie S Bauerfeind, Klaus Fischer

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Citations

Nov 28, 2017·Scientific Reports·Timothée BrütschMichel Chapuisat
Jan 10, 2016·The Journal of Experimental Biology·Shelley A AdamoKurtis F Turnbull
Aug 13, 2017·Scientific Reports·Maximilian KörnerJoël Meunier
Apr 12, 2020·Applied and Environmental Microbiology·Luis R Paniagua VoirolMonika Hilker
Apr 1, 2020·International Journal for Parasitology·Francisco Arcila, Joël Meunier

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