Podophyllotoxin lignans enhance IL-1beta but suppress TNF-alpha mRNA expression in LPS-treated monocytes

Immunopharmacology and Immunotoxicology
N PughD S Pasco

Abstract

There exists a growing body of research which indicates that antimitotics such as taxol and colchicine influence cytokine gene expression. In the present study we examined the effect of podophyllotoxin and six analogs on nuclear factor kappa B (NF-kappa B) activation, and on interleukin-1 beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) mRNA expression in human THP-1 monocytes. All compounds were inactive between 0.001microM and 10microM when tested alone. However, podophyllotoxin (0.1 microM) enhanced LPS-induced NF-kappa B activation and IL-1beta mRNA expression between 2 and 3-fold. In contrast, LPS-induced TNF-alpha mRNA expression was decreased between 3 and 6-fold. Comparable results were also observed with the three analogs acetylpodophyllotoxin, 4'-demethylpodophyllotoxin and alpha-peltatin. The remaining three analogs (podophyllotoxin-4-O-glucoside, beta-peltatin-beta-D-glucopyransoide and 1,2,3,4-dehydrodesoxypodophyllotoxin) were inactive. Clearly certain structural features such as the presence of a glycosidic group or ring aromatization results in loss of biological activity. Interestingly, the analogs that were inactive in our assays have also been previously shown to lack affinity for tubulin binding. ...Continue Reading

References

Jan 1, 1987·International Journal of Immunopharmacology·Q Y ZhengJ W Hadden
Feb 5, 1998·Archives of Dermatology·S TyringT Toter
Mar 24, 1998·Immunology Today·M J May, S Ghosh
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A ByrdA Ding
May 2, 2000·Rheumatology·T Lerndal, B Svensson

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Citations

Dec 27, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M Gordaliza
Dec 19, 2006·Evidence-based Complementary and Alternative Medicine : ECAM·Raman ChawlaG N Qazi
Mar 5, 2011·Biomedical Chromatography : BMC·Bharathi AvulaIkhlas A Khan
May 16, 2014·Medicinal Research Reviews·Ying-Qian LiuKuo-Hsiung Lee
Mar 17, 2017·Journal of Cutaneous Medicine and Surgery·Sophia Colantonio, Jason K Rivers
Dec 16, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marina Pereira RochaClaudia Gontijo Silva

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