Podospora anserina target of rapamycin.

Current Genetics
Bérangère Pinan-LucarréCorinne Clavé

Abstract

We have isolated the Podospora anserina TOR gene. The PaTOR protein displayed strong identities with TOR proteins from other eukaryotes especially in the FRB domain and the kinase domain. Genome analysis suggests that a single TOR gene exists in Podospora. The serine residue known to be one site of missense mutations conferring rapamycin resistance in other organisms is conserved in the PaTOR protein (S1895). A PaTOR-S1895R mutated allele has been constructed and introduced in the wild-type strain, as expected strains expressing the PaTOR-S1895R gene become resistant to rapamycin. The dominance of the PaTOR-S1895R allele indicates that apparently the mutation does not impair the kinase activity. We confirm that all cytological modifications associated with rapamycin treatment in Podospora are indeed mediated by PaTOR. We conclude that the PaTOR gene is likely to be essential and that rapamycin treatment might be useful to further investigate rapamycin-sensitive TOR functions in Podospora and especially newly identified rapamycin-sensitive functions such as the autophagy-independent control of vacuole remodeling and septation.

References

Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J HeitmanM N Hall
May 1, 1989·Virology·R J Russell, S J Robbins
Nov 17, 1995·The Journal of Biological Chemistry·M C Lorenz, J Heitman
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J ChenS L Schreiber
Oct 1, 1995·Nature Genetics·M A Andrade, P Bork
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·M I ChiuV Berlin
Feb 1, 1994·Current Genetics·G Lecellier, P Silar
Jan 1, 1996·Molecular Biology of the Cell·N C BarbetM N Hall
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SchmidtM N Hall
Oct 7, 1997·Molecular and Cellular Biology·C M Alarcón, J Heitman
Apr 27, 2000·Trends in Biochemical Sciences·R BosottiE L Sonnhammer
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·S J Saupe
Nov 9, 2000·Genes & Development·H ZhangT P Neufeld
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Benoît MenandChristophe Robaglia
Apr 10, 2003·Eukaryotic Cell·Karine DementhonCorinne Clavé
May 14, 2003·Transplantation Proceedings·S N Sehgal
Oct 7, 2004·Nature Cell Biology·Estela JacintoMichael N Hall
Sep 10, 2005·Eukaryotic Cell·Gregory J FitzgibbonMark X Caddick
Nov 10, 2005·Eukaryotic Cell·Bérangère Pinan-LucarréCorinne Clavé

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Citations

Oct 1, 2013·Applied Microbiology and Biotechnology·Oliver Voigt, Stefanie Pöggeler
Sep 30, 1996·Mechanisms of Ageing and Development·M Rossignol, P Silar
Aug 18, 2010·Antioxidants & Redox Signaling·Magdalena Bartoszewska, Jan A K W Kiel
Jan 15, 2016·Mechanisms of Ageing and Development·Cihan Suleyman ErdoganOle Vang
Jan 1, 2009·Fungal Genetics and Biology : FG & B·Heber Gamboa MeléndezGéraldine Mey
Jan 6, 2007·Seminars in Cancer Biology·Bérangère Pinan-LucarréCorinne Clavé
Sep 1, 2012·Autophagy·Xiao-Hong LiuFu-Cheng Lin
Sep 6, 2011·Fungal Genetics and Biology : FG & B·M A van der NestB D Wingfield

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