Point mutation detection by surface plasmon resonance imaging coupled with a temperature scan method in a model system

Analytical Chemistry
J B FicheThierry Livache

Abstract

The detection of point mutations in genes presents clear biological and medical interest. Various methods have been considered. In this paper, we take advantage of surface plasmon resonance imaging, a technique allowing detection of unlabeled DNA hybridization. Coupled with a temperature scan, this approach allows us to determine the presence of single-point mutations in oligonucleotide samples from the analysis of DNA's melting curves in either the homozygous or heterozygous case. Moreover, these experimental data are confirmed in good agreement with numerical calculations.

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Citations

Nov 29, 2012·Analytical and Bioanalytical Chemistry·Roberta D'Agata, Giuseppe Spoto
Jan 12, 2013·Biosensors & Bioelectronics·Wei ShenZhiqiang Gao
Jan 12, 2013·Nucleic Acids Research·Andrew HarrisonAlexander E Pozhitkov
Feb 26, 2014·Analytical and Bioanalytical Chemistry·Stefano Mariani, Maria Minunni
Oct 3, 2009·Analytical Biochemistry·Julia FuchsThierry Livache
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Dec 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Tyler J PettyAric Opdahl
Jun 5, 2013·Bioconjugate Chemistry·F MallevreT Livache
Mar 18, 2009·Biochemical Society Transactions·Alexander Chagovetz, Steve Blair
Aug 6, 2013·Lab on a Chip·Sihem BougueliaThierry Livache
Jan 12, 2011·Chemical Society Reviews·Serge Cosnier, Michael Holzinger
Oct 8, 2016·Nanoscale·Feriel MelaineArnaud Buhot
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Feb 21, 2009·Analytical Chemistry·Niel CrewsBruce Gale
Feb 18, 2017·Analytical Chemistry·Lucyano J A MacedoAric Opdahl

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